Detailed gene copy number and RNA expression analysis of the 17q12–23 region in primary breast cancers
Article first published online: 30 JAN 2003
Copyright © 2003 Wiley-Liss, Inc.
Genes, Chromosomes and Cancer
Volume 36, Issue 4, pages 382–392, April 2003
How to Cite
Willis, S., Hutchins, A.-M., Hammet, F., Ciciulla, J., Soo, W.-K., White, D., van der Spek, P., Henderson, M. A., Gish, K., Venter, D. J. and Armes, J. E. (2003), Detailed gene copy number and RNA expression analysis of the 17q12–23 region in primary breast cancers. Genes Chromosom. Cancer, 36: 382–392. doi: 10.1002/gcc.10138
- Issue published online: 24 FEB 2003
- Article first published online: 30 JAN 2003
- Manuscript Accepted: 25 JUN 2002
- Manuscript Received: 28 MAR 2002
Chromosome region 17q12–23 commonly shows an increase in DNA copy number in breast cancers, suggesting that several oncogenes are located at this site. We performed a high-resolution expression array and comparative genomic hybridization analysis of genes mapped to the entire 17q12–23 region, to identify novel candidate oncogenes. We identified 24 genes that showed significant overexpression in breast cancers with gain of 17q12–23, compared to cancers without gain. These genes included previously identified oncogenes, together with several novel candidate oncogenes. FISH analysis using specific gene probes hybridized to tissue arrays confirmed the underlying amplification of overexpressed genes. This high-resolution analysis of the 17q12–23 region indicates that several established and novel candidate oncogenes, including a Wnt-signaling pathway member, are amplified and overexpressed within individual primary breast cancer samples. We were also able to confirm the presence of two apparently separate and reciprocally amplified groups of genes within this region. Investigation of these genes and their functional interactions will facilitate our understanding of breast oncogenesis and optimal management of this disease. © 2003 Wiley-Liss, Inc.