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Detailed gene copy number and RNA expression analysis of the 17q12–23 region in primary breast cancers

Authors

  • Simon Willis,

    1. Molecular Pathology Laboratory, Victorian Breast Cancer Research Consortium, University of Melbourne and Peter MacCallum Cancer Institute, Melbourne, Australia
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  • Anne-Marie Hutchins,

    1. Molecular Pathology Laboratory, Victorian Breast Cancer Research Consortium, University of Melbourne and Peter MacCallum Cancer Institute, Melbourne, Australia
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  • Fleur Hammet,

    1. Molecular Pathology Laboratory, Victorian Breast Cancer Research Consortium, University of Melbourne and Peter MacCallum Cancer Institute, Melbourne, Australia
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  • John Ciciulla,

    1. Cancer Genomics Laboratory, Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Australia
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  • Wee-Kheng Soo,

    1. Cancer Genomics Laboratory, Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Australia
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  • David White,

    1. Molecular Pathology Laboratory, Victorian Breast Cancer Research Consortium, University of Melbourne and Peter MacCallum Cancer Institute, Melbourne, Australia
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  • Peter van der Spek,

    1. Bioinformatics Department, Johnson & Johnson Pharmaceutical Research and Development, Beerse, Belgium
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  • Michael A. Henderson,

    1. Department of Surgical Oncology, Peter MacCallum Cancer Institute and Department of Surgery, University of Melbourne, Melbourne, Australia
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  • Kurt Gish,

    1. Eos Biotechnology, South San Francisco, California
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  • Deon J. Venter,

    1. Cancer Genomics Laboratory, Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Australia
    2. Department of Pathology, Peter MacCallum Cancer Institute, Melbourne, Australia
    3. Department of Pathology, University of Melbourne, Melbourne, Australia
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  • Jane E. Armes

    Corresponding author
    1. Molecular Pathology Laboratory, Victorian Breast Cancer Research Consortium, University of Melbourne and Peter MacCallum Cancer Institute, Melbourne, Australia
    2. Department of Pathology, Peter MacCallum Cancer Institute, Melbourne, Australia
    3. Department of Pathology, University of Melbourne, Melbourne, Australia
    4. Department of Pathology, Royal Women's Hospital and Melbourne Pathology, Melbourne, Australia
    • Royal Women's Hospital, Department of Pathology, Grattan Street, Carlton, Victoria 3053, Australia
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Abstract

Chromosome region 17q12–23 commonly shows an increase in DNA copy number in breast cancers, suggesting that several oncogenes are located at this site. We performed a high-resolution expression array and comparative genomic hybridization analysis of genes mapped to the entire 17q12–23 region, to identify novel candidate oncogenes. We identified 24 genes that showed significant overexpression in breast cancers with gain of 17q12–23, compared to cancers without gain. These genes included previously identified oncogenes, together with several novel candidate oncogenes. FISH analysis using specific gene probes hybridized to tissue arrays confirmed the underlying amplification of overexpressed genes. This high-resolution analysis of the 17q12–23 region indicates that several established and novel candidate oncogenes, including a Wnt-signaling pathway member, are amplified and overexpressed within individual primary breast cancer samples. We were also able to confirm the presence of two apparently separate and reciprocally amplified groups of genes within this region. Investigation of these genes and their functional interactions will facilitate our understanding of breast oncogenesis and optimal management of this disease. © 2003 Wiley-Liss, Inc.

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