Consistent chromosome 10 rearrangements in four newly established human hepatocellular carcinoma cell lines
Article first published online: 16 NOV 2001
Copyright © 2002 Wiley-Liss, Inc.
Genes, Chromosomes and Cancer
Volume 33, Issue 2, pages 150–159, February 2002
How to Cite
Pang, E., Wong, N., Lai, P. B.-S., To, K.-F., Lau, W.-Y. and Johnson, P. J. (2002), Consistent chromosome 10 rearrangements in four newly established human hepatocellular carcinoma cell lines. Genes Chromosom. Cancer, 33: 150–159. doi: 10.1002/gcc.1220
- Issue published online: 21 DEC 2001
- Article first published online: 16 NOV 2001
- Manuscript Accepted: 31 JUL 2001
- Manuscript Received: 27 MAR 2001
- The Kadoorie Charitable Foundations, Hong Kong
- Providence Foundation Limited, Hong Kong
Cancer cell lines represent an invaluable resource for isolation of novel genes relevant to tumor pathogenesis and as in vitro models. In this study, we report on the successful establishment of four cell lines from hepatocellular carcinoma tissue. These cell lines, designated HKCI-1, HKCI-2, HKCI-3, and HKCI-4, have been growing continuously for more than 24 months and have been passaged more than 50 times. A comprehensive cytogenetic characterization on the primary tumors and the derived cell lines was achieved by the combined approach of spectral karyotyping and comparative genomic hybridization. Chromosomal imbalances from the primary tumors were also maintained in the cell lines and included gains of 1q, 6p, 7, 10p, 17q, and 20 and loss of 4q. Recurring translocations included t(X;11), t(1;10), t(4;16), i(5)(p10), t(7;21), t(8;17), t(9;22), i(10)(p10), t(14;20), t(16;22), and t(17;19). It was noteworthy that consistent chromosome 10 aberrations, in particular t(1;10)(q10;p10), were detected in all four cell lines. Furthermore, microsatellite analysis on primary tumor and derived cell lines indicated a common deleted region of 10q23-q26. The functional importance of chromosome 10 aberrations in relation to the pathogenesis of HCC is unknown; however, the high frequency with which such aberrations were maintained in the cell lines suggests proliferative advantages of 10q loss or 10p gain in the multistep development of hepatic neoplasia. © 2002 Wiley-Liss, Inc.