Frequent alterations in the Wnt signaling pathway in colorectal cancer with microsatellite instability
Article first published online: 6 DEC 2001
Copyright © 2002 Wiley-Liss, Inc.
Genes, Chromosomes and Cancer
Volume 33, Issue 1, pages 73–81, January 2002
How to Cite
Shimizu, Y., Ikeda, S., Fujimori, M., Kodama, S., Nakahara, M., Okajima, M. and Asahara, T. (2002), Frequent alterations in the Wnt signaling pathway in colorectal cancer with microsatellite instability. Genes Chromosom. Cancer, 33: 73–81. doi: 10.1002/gcc.1226
- Issue published online: 6 DEC 2001
- Article first published online: 6 DEC 2001
- Manuscript Accepted: 5 JUL 2001
- Manuscript Received: 2 APR 2001
- Tsuchiya Memorial Foundation
It is generally accepted that both dysfunction of the Wnt signaling pathway, including mutations in the adenomatous polyposis coli (APC) and β-catenin genes, and genetic instability play important roles in colorectal carcinogenesis. However, alteration of the components in the Wnt signaling pathway in colorectal cancer (CRC) with microsatellite instability (MSI) has not been elucidated. In order to assess the status of the Wnt signaling components in CRC with MSI, mutational analyses of the β-catenin, APC, Axin 1, and T cell factor 4 (TCF4) genes were performed. Three of 33 samples had mutations in exon 3 of the β-catenin gene and two in the APC gene. Eight mutations in seven samples were detected by single-strand conformation polymorphism and subsequent direct sequence analysis of the entire coding region of the Axin 1 gene. Furthermore, TCF4, which is one of the transcriptional factors in the Wnt signaling pathway and has a mononucleotide repeat sequence (a nine- adenine repeat, (A)9) in its C-terminal region, was mutated in 13 of the 33 samples. Thus, alteration in the Wnt signaling pathway is frequently observed in CRC with MSI, including hereditary nonpolyposis colorectal cancer, as well as in familial adenomatous polyposis and sporadic CRC without MSI. © 2002 Wiley-Liss, Inc.