Induced DNA recombination by Cre recombinase protein transduction
Version of Record online: 25 APR 2002
Copyright © 2002 Wiley-Liss, Inc.
Volume 33, Issue 1, pages 48–54, May 2002
How to Cite
Joshi, S. K., Hashimoto, K. and Koni, P. A. (2002), Induced DNA recombination by Cre recombinase protein transduction. Genesis, 33: 48–54. doi: 10.1002/gene.10089
- Issue online: 25 APR 2002
- Version of Record online: 25 APR 2002
- Manuscript Accepted: 16 MAR 2002
- Manuscript Received: 6 DEC 2001
- Cre recombinase;
- protein transduction;
- induced DNA recombination;
- gene deletion
Summary: Cre is a DNA recombinase that recognizes 34 base-pair loxP sites of recombination. We have developed a cell-permeable Cre recombinase, TATCre, that is capable of mediating deletion of loxP-flanked targets by simply adding TATCre to cell cultures. Thus, TATCre allows efficient induced DNA recombination without the use of a Cre recombinase transgene or any other genetic material and should prove useful for the genetic manipulation of a wide variety of cell types that have been engineered to possess loxP sites. genesis 33:48–54, 2002. © 2002 Wiley-Liss, Inc.