Expression of Cre recombinase in the developing mouse limb bud driven by a Prxl enhancer
Article first published online: 8 MAY 2002
Copyright © 2002 Wiley-Liss, Inc.
Volume 33, Issue 2, pages 77–80, June 2002
How to Cite
Logan, M., Martin, J. F., Nagy, A., Lobe, C., Olson, E. N. and Tabin, C. J. (2002), Expression of Cre recombinase in the developing mouse limb bud driven by a Prxl enhancer. Genesis, 33: 77–80. doi: 10.1002/gene.10092
- Issue published online: 8 MAY 2002
- Article first published online: 8 MAY 2002
- National Institutes of Health. Grant Number: HD32433
- NIDCR. Grant Numbers: R29 DE12324, R01DE013509
- March of Dimes. Grant Number: 5-FY00-135
Summary: We have used a Prx1 limb enhancer to drive expression of Cre Recombinase in transgenic mice. This regulatory element leads to Cre expression throughout the early limb bud mesenchyme and in a subset of craniofacial mesenchyme. Crossing a murine line carrying this transgene to a reporter mouse harboring a floxed Cre-reporter cassette revealed that recombinase activity is first observed in the earliest limb bud at 9.5 dpc. By early to mid bud stages at 10.5 dpc recombination is essentially complete in all mesenchymal cells in the limb. Expression of the Cre recombinase was never detected in the limb bud ectoderm. The use of Prx1–Cre mice should facilitate analysis of gene function in the developing limb. genesis 33:77–80, 2002. © 2002 Wiley-Liss, Inc.