In utero complementation of a neural crest-derived melanocyte defect using cell directed gene transfer
Version of Record online: 8 JUN 2001
Copyright © 2001 Wiley-Liss, Inc.
Volume 30, Issue 2, pages 70–76, June 2001
How to Cite
Dunn, K. J., Incao, A., Watkins-Chow, D., Li, Y. and Pavan, W. J. (2001), In utero complementation of a neural crest-derived melanocyte defect using cell directed gene transfer. Genesis, 30: 70–76. doi: 10.1002/gene.1035
- Issue online: 8 JUN 2001
- Version of Record online: 8 JUN 2001
- Manuscript Accepted: 1 MAY 2001
- Manuscript Received: 9 MAR 2001
- neural crest;
Summary: This study describes an in utero approach for overexpressing genes in a cell-type directed manner. It uses an avian leukosis retroviral expression system coupled with a transgenic mouse line expressing the viral receptor tv-a from a tissue-specific promoter (RCAS-TVA system) (Federspiel et al., 1994, and reviewed in Fisher et al., 1999). A transgenic mouse line was generated expressing tv-a from the Dopachrome tautomerase promoter (DCT-tv-a) in embryonic melanocyte precursors (melanoblasts). RCAS virus encoding β-galactosidase (RCAS-LacZ) or tyrosinase (RCAS-Tyr) was injected in utero into embryonic day 12.5 albino (tyrosinase inactive) mouse embryos. Animals were analyzed for β-galactosidase activity or tyrosinase activity (hair pigmentation). RCAS gene expression was detected in 44% and 25% of the transgenic mice, respectively. We demonstrate the RCAS-TVA system coupled with the DCT-tv-a line of mice can be used for in utero infection. genesis 30:70–76, 2001. © 2001 Wiley-Liss, Inc.