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Action of morpholinos in Ciona embryos

  1. Yutaka Satou*,
  2. Kaoru S. Imai,
  3. Nori Satoh

Article first published online: 23 JUL 2001

DOI: 10.1002/gene.1040

Issue

genesis

genesis

Volume 30, Issue 3, pages 103–106, July 2001

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How to Cite

Satou, Y., Imai, K. S. and Satoh, N. (2001), Action of morpholinos in Ciona embryos. Genesis, 30: 103–106. doi: 10.1002/gene.1040

Author Information

  1. Department of Zoology, Graduate School of Science, Kyoto University, Kyoto, Japan

*Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan

Publication History

  1. Issue published online: 23 JUL 2001
  2. Article first published online: 23 JUL 2001
  3. Manuscript Accepted: 9 MAY 2001
  4. Manuscript Received: 26 FEB 2001

Funded by

  • Grant-in-aids from Monbusho, Japan
  • Uehara Foundation

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Abstract

Summary: First, using morpholino against lacZ, we demonstrate that the morpholino specifically suppresses the translation of the gene introduced exogenously into Ciona eggs. Second, using morpholino against an alkaline phosphatase gene, we show that the morpholino suppresses the translation of the endogenous gene as well. Third, using morpholino against β-catenin gene, we confirm that the suppression by the morpholino can be rescued by injection of β-catenin mRNA. All of these results indicate that morpholino act in Ciona embryos to specifically block the function of endogenous genes as well as exogenously introduced genes. genesis 30: 103–106, 2001. © 2001 Wiley-Liss, Inc.

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