Article

You have free access to this content

Morpholino-induced knockdown of zebrafish engrailed genes eng2 and eng3 reveals redundant and unique functions in midbrain–hindbrain boundary development

  1. Steffen Scholpp,
  2. Michael Brand*

Article first published online: 23 JUL 2001

DOI: 10.1002/gene.1047

Issue

genesis

genesis

Volume 30, Issue 3, pages 129–133, July 2001

Additional Information

How to Cite

Scholpp, S. and Brand, M. (2001), Morpholino-induced knockdown of zebrafish engrailed genes eng2 and eng3 reveals redundant and unique functions in midbrain–hindbrain boundary development. Genesis, 30: 129–133. doi: 10.1002/gene.1047

Author Information

  1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

*Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany

Publication History

  1. Issue published online: 23 JUL 2001
  2. Article first published online: 23 JUL 2001
  3. Manuscript Accepted: 18 JUN 2001
  4. Manuscript Received: 31 MAY 2001

Funded by

  • Deutsche Forschungsgemeinschaft, Graduiertenkolleg Entwicklungsbiologie 484, EU–Biotech-Program,
  • Max-Planck-Society

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (483K)

LITERATURE CITED

  • Davis CA, Holmyard DP, Millen KJ, Joyner AL. 1991. Examining pattern formation in mouse, chicken and frog embryos with an En-specific antiserum. Development 111: 287–298.
  • Ekker M, Akimenko MA, Bremiller R, Westerfield M. 1992. Regional expression of three homeobox transcripts in the inner ear of zebrafish embryos. Neuron 9: 27–35.
  • Fjose A, Eiken HG, Njolstad PR, Molven A, Hordvik I. 1988. A zebrafish engrailed-like homeobox sequence expressed during embryogenesis. FEBS Lett 231: 355–360.
  • Force A, Lynch M, Pickett FB, Amores A, Yan YL, Poslethwait J. 1999. Preservation of duplicate genes by complementary, degenerative mutations. Genetics 151: 1532–1545.
  • Holland PW, Williams, NA. 1990. Conservation of engrailed-like homeobox sequences during vertebrate evolution. FEBS Lett 277: 250–252.
  • Joyner AL, Martin GR. 1987. En-1 and En-2, two mouse genes with sequence homology to the Drosophila engrailed gene: expression during embryogenesis. Genes Dev 1: 29–38.
  • Lun K, Brand M. 1998. A series of no isthmus (noi) alleles of the zebrafish pax2.1 gene reveals multiple signaling events in development of the midbrain-hindbrain boundary. Development 125: 3049–3062.
  • Nasevicius A, Ekker SC. 2000. Effective targeted gene ‘knockdown‘ in zebrafish. Nat Genet 26: 216–220.
  • Pfeffer PL, Bouchard M, Busslinger M. 2000. Pax2 and homeodomain proteins cooperatively regulate a 435bp enhancer of the mouse Pax5 gene at the midbrain-hindbrain boundary. Development 127: 1017–1028.
  • Postlethwait JH, Yan YL, Gates MA, Horne S, Amores A, Brownlie A, Donovan A, Egan ES, Force A, Gong Z, Goutel C, Fritz A, Kelsh R, Knapik E, Liao E, Paw B, Ransom D, Singer A, Thomson M, Abduljabbar TS, Yelick P, Beier D, Joly JS, Larhammar D, Rosa F, 1998. Vertebrate genome evolution and the zebrafish gene map. Nat Genet 18: 345–349.
  • Song DL, Chalepakis G, Gruss P, Joyner AL. 1996. Two Pax-binding sites are required for early embryonic brain expression of an Engrailed-2 transgene. Development 122: 627–635.
Get PDF (483K)