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Pitx1 and Pitx2c are required for ectopic cement gland formation in xenopus laevis

  1. Axel Schweickert1,
  2. Kirsten Deißler1,
  3. Martin Blum1,*,
  4. Herbert Steinbeisser2

Article first published online: 23 JUL 2001

DOI: 10.1002/gene.1051

Issue

genesis

genesis

Volume 30, Issue 3, pages 144–148, July 2001

Additional Information

How to Cite

Schweickert, A., Deißler, K., Blum, M. and Steinbeisser, H. (2001), Pitx1 and Pitx2c are required for ectopic cement gland formation in xenopus laevis. Genesis, 30: 144–148. doi: 10.1002/gene.1051

Author Information

  1. 1

    Forschungszentrum Karlsruhe, Institute of Toxicology and Genetics, Karlsruhe, Germany

  2. 2

    Max-Planck-Institut für Entwicklungsbiologie, Abt. Zellbiologie, Tübingen, Germany

*Forschungszentrum Karlsruhe, Institute of Genetics, PO Box 3640, D-76021 Karlsruhe, Germany

Publication History

  1. Issue published online: 23 JUL 2001
  2. Article first published online: 23 JUL 2001
  3. Manuscript Accepted: 16 MAY 2001
  4. Manuscript Received: 24 APR 2001

Funded by

  • DFG (BL 285/4-1)
  • Volkswagenstiftung

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Keywords:

  • Xenopus;
  • homeobox genes;
  • Pitx1;
  • Pitx2c;
  • cement gland;
  • morpholino oligos

Abstract

Summary: The mucus secreting cement gland is the anterior-most ectodermal organ of the Xenopus embryo. The homeobox genes Pltx1 and Pitx2c are expressed in the cement gland primordium. Misexpression of both genes induced ectopic cement gland tissue in whole embryos and transcription of the marker genes Xag1 and Xag2 in animal cap explant cultures. Antisense morpholino oligonucleotides against Pitx1 and Pitx2c inhibited ectopic cement gland formation induced by otx2. Gene knock downs generated by morpholino oligonucleotides were specific and could be rescued by coinjection of Pitx mRNAs. These data demonstrate for the first time the requirement of specific genes for cement gland formation by loss-of-function experiments. genesis 30:144–148, 2001. © 2001 Wiley-Liss, Inc.

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