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A zebrafish vasa morphant abolishes Vasa protein but does not affect the establishment of the germline

  1. Arie K. Braat†,
  2. Sandra van de Water,
  3. Jeroen Korving,
  4. Danica Zivkovic*

Article first published online: 23 JUL 2001

DOI: 10.1002/gene.1060

Issue

genesis

genesis

Volume 30, Issue 3, pages 183–185, July 2001

Additional Information

How to Cite

Braat, A. K., van de Water, S., Korving, J. and Zivkovic, D. (2001), A zebrafish vasa morphant abolishes Vasa protein but does not affect the establishment of the germline. Genesis, 30: 183–185. doi: 10.1002/gene.1060

Author Information

  1. Hubrecht Laboratory, The Netherlands Institute for Developmental Biology, Utrecht, The Netherlands

  1. †

    Institute for Cellular and Molecular Biology, Section of Molecular Cell and Developmental Biology, The University of Texas at Austin, 2500 Speedway, Austin, TX 78712-1095

*Hubrecht Laboratory, The Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands

Publication History

  1. Issue published online: 23 JUL 2001
  2. Article first published online: 23 JUL 2001
  3. Manuscript Accepted: 9 MAY 2001
  4. Manuscript Received: 27 APR 2001

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LITERATURE CITED

  • Braat AK, Water van de S, Goos H, Bogerd J, Zivkovic D. 2000. Vasa protein expression and localization in the zebrafish. Mech Dev 95: 271–274.
  • Braat AK, Zandbergen T, Water van de S, Goos HJTh, Zivkovic D. 1999. Characterization of zebrafish primordial germ cells: Morphology and early distribution of vasa RNA. Dev Dyn 216: 153–167.
    Direct Link:
  • Fraser AG, Kamath RS, Zipperlen P, Martinez-Campos M, Sohrmann M, Ahringer J. 2000. Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408: 325–330.
  • Gruidl ME, Smith PA, Kuznicki KA, McCrone JS, Kirchner J, Roussell DL, Bennett K. 1996. Multiple potential germ-line helicases are components of the germ-line specific P granules of Caenorhabditis elegans. Proc Natl Acad Sci USA 93: 13837–13842.
  • Hay B, Ackerman L, Barbel S, Jan LY, Jan YN. 1988a. Identification of a component of Drosophila polar granules. Development 103: 625–640.
  • Hay B, Jan LY, Jan YN. 1988b. A protein component of Drosophila polar granules is encoded by vasa and has extensive sequence similarity to ATP-dependent helicases. Cell 55: 577–587.
  • Ikenishi K, Tanaka TS. 1997. Involvement of the protein of Xenopus vasa homolog (Xenopus vasa-like gene 1, XVLG1) in the differentiation of primordial germ cells. Dev Growth Differ 39: 625–633.
    Direct Link:
  • Knaut H, Pelegri F, Bohmann K, Schwarz H, Nusslein-Volhard C. 2000. Zebrafish vasa RNA but not its protein is a component of the germ plasm and segregates asymmetrically before germline specification. J Cell Biol 149: 875–888.
  • Kuznicki KA, Smith PA, Leung-Chiu WMA, Estevez AO, Scott HC, Bennett KL. 2000. Combinatorial RNA interference indicates GLH-4 can compensate for GLH-1; these two P granule components are critical for fertility in C. elegans. Development 127: 2907–2916.
  • Lasko PF, Ashburner M. 1988. The product of the Drosophila gene vasa is very similar to eukaryotic initiation factor-4A. Nature 335: 611–617.
  • Misquitta L, Paterson BM. 1999. Targeted disruption of gene function in Drosophila by RNA interference (RNA-i): A role for nautilus in embryonic somatic muscle formation. Proc Natl Acad Sci USA 96: 1451–1456.
  • Oates AC, Bruce AE, Ho RK. 2000. Too much interference: Injection of double-stranded RNA has nonspecific effects in the zebrafish embryo. Dev Biol 224: 20–28.
  • Olsen LC, Aasland R, Fjose A. 1997. A vasa-like gene in zebrafish identifies putative primordial germ cells. Mech Dev 66: 95–105.
  • Wargelius A, Ellingsen S, Fjose A. 1999. Double-stranded RNA induces specific developmental defects in zebrafish embryos. Biochem Biophys Res Commun 263: 156–161.
  • Yoon C, Kawakami K, Hopkins N. 1997. Zebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4-cell stage embryos and is expressed in the primordial germ cells. Development 124: 3157–3165.
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