Letter

A keratin K5Cre transgenic line appropriate for tissue-specific or generalized cre-mediated recombination

  1. Angel Ramirez1,†,
  2. Angustias Page1,
  3. Alberto Gandarillas2,
  4. Jennifer Zanet2,†,
  5. Sophie Pibre2,†,
  6. Miguel Vidal3,
  7. Laura Tusell4,
  8. Anna Genesca4,
  9. Duncan A. Whitaker5,
  10. David W. Melton6,
  11. Jose L. Jorcano1,*

Article first published online: 28 APR 2004

DOI: 10.1002/gene.20025

Issue

genesis

genesis

Volume 39, Issue 1, pages 52–57, May 2004

Additional Information

How to Cite

Ramirez, A., Page, A., Gandarillas, A., Zanet, J., Pibre, S., Vidal, M., Tusell, L., Genesca, A., Whitaker, D. A., Melton, D. W. and Jorcano, J. L. (2004), A keratin K5Cre transgenic line appropriate for tissue-specific or generalized cre-mediated recombination. Genesis, 39: 52–57. doi: 10.1002/gene.20025

Author Information

  1. 1

    Epithelial Damage, Repair and Tissue Engineering Program, CIEMAT, Madrid, Spain

  2. 2

    Institut de Génétique Moleculaire de Montpellier, CNRS/UMII, Montpellier, France

  3. 3

    Department of Developmental and Cell Biology, CIB, CSIC, Madrid, Spain

  4. 4

    Department of Cell Biology, Physiology and Immunology, School of Medicine, University Autonoma of Barcelona, Bellaterra, Spain

  5. 5

    MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland, UK

  6. 6

    Sir Alastair Currie Cancer Research UK Laboratories, Molecular Medicine Centre, University of Edinburgh, Western General Hospital, Edinburgh, Scotland, UK

  1. †

    Laboratoire de Dermatologie Moléculaire. Institut Universitaire de Recherche Clinique, UMPI, Montpellier, France

*Epithelial Damage, Repair and Tissue Engineering Program, CIEMAT, Av. Complutense 22, 28040 Madrid, Spain

Publication History

  1. Issue published online: 28 APR 2004
  2. Article first published online: 28 APR 2004
  3. Manuscript Accepted: 10 FEB 2004
  4. Manuscript Received: 25 AUG 2003

Funded by

  • Medical Research Council
  • Spanish Ministry of Science and Technology
  • La Ligue National contre le Cancer and l'ARC (France)
  • Cancer Research UK

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Keywords:

  • Cre recombinase;
  • tissue-specific recombination;
  • epidermis;
  • keratinocyte;
  • maternal deleter;
  • transgenic mice

Abstract

We describe here a mouse line bearing a bovine keratin K5Cre recombinase transgene. These mice showed a dual pattern of Cre-mediated recombination, depending on the parent transmitting the transgene. In paternal transmission, recombination occurred specifically in the skin and stratified epithelia—as expected according to the expression of endogenous keratin K5. However, constitutive recombination between loxP sites transmitted by the sperm took place when the mother possessed the K5Cre transgene, even when the transgene was absent in the progeny. Cre expression in late-stage oocytes, with the Cre protein persisting into the developing embryo, leads to the constitutive recombination observed. Thus, this transgenic line allows for both tissue-specific and generalized recombination, depending on the breeding scheme. genesis 39:52–57, 2004. © 2004 Wiley-Liss, Inc.

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