Lentivirally generated eGFP-transgenic rats allow efficient cell tracking in vivo
Article first published online: 25 MAY 2004
Copyright © 2004 Wiley-Liss, Inc.
Volume 39, Issue 2, pages 94–99, June 2004
How to Cite
van den Brandt, J., Wang, D., Kwon, S.-H., Heinkelein, M. and Reichardt, H. M. (2004), Lentivirally generated eGFP-transgenic rats allow efficient cell tracking in vivo. Genesis, 39: 94–99. doi: 10.1002/gene.20037
- Issue published online: 25 MAY 2004
- Article first published online: 25 MAY 2004
- Manuscript Accepted: 15 MAR 2004
- Manuscript Received: 12 DEC 2003
- eGFP-transgenic rats;
Here we describe the efficient generation of eGFP-transgenic rats using a lentiviral approach. Analysis of the founder generation demonstrated that 46% of the offspring had stably integrated the provirus into the genome and of those 92% expressed eGFP in all blood-derived leukocytes. In contrast to their offspring, all founder rats were mosaic with regard to eGFP-expression, suggesting delayed viral transduction after injection. The expression level of eGFP in the F1 generation is influenced by and segregates with the site of proviral integration. Interestingly, a single copy of the transgene is sufficient for reliable detection by flow cytometry, irrespective of the leukocyte subtype analyzed. Adoptive transfer of purified CD4+ T-lymphocytes from transgenic rats and subsequent reisolation from various organs further demonstrated that expression of the lentiviral transgene is maintained in a foreign host and therefore allows for efficient tracking of transferred cells. Taken together, lentivirally generated eGFP-transgenic rats are a powerful tool for various applications in immunology and presumably also many other fields. genesis 39:94–99, 2004. © 2004 Wiley-Liss, Inc.