Article

Gene expression profile analysis of mouse colon embryonic development

  1. Young-Kyu Park1,
  2. Jeffrey L. Franklin2,
  3. Stephen H. Settle1,
  4. Shawn E. Levy3,
  5. Eunkyung Chung1,
  6. Loice H. Jeyakumar1,
  7. Yu Shyr4,
  8. Mary Kay Washington5,
  9. Robert H. Whitehead1,
  10. Bruce J. Aronow6,
  11. Robert J. Coffey1,2,7,*

Article first published online: 11 JAN 2005

DOI: 10.1002/gene.20088

Issue

genesis

genesis

Volume 41, Issue 1, pages 1–12, January 2005

Additional Information

How to Cite

Park, Y.-K., Franklin, J. L., Settle, S. H., Levy, S. E., Chung, E., Jeyakumar, L. H., Shyr, Y., Washington, M. K., Whitehead, R. H., Aronow, B. J. and Coffey, R. J. (2005), Gene expression profile analysis of mouse colon embryonic development. Genesis, 41: 1–12. doi: 10.1002/gene.20088

Author Information

  1. 1

    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee

  2. 2

    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee

  3. 3

    Department of Biomedical Informatics, Vanderbilt University School of Medicine, Nashville, Tennessee

  4. 4

    Department of Preventive Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee

  5. 5

    Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee

  6. 6

    Division of Developmental Biology and Division of Pediatric Informatics, Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio

  7. 7

    Department of Veterans Affairs Medical Center, Nashville, Tennessee

*Suite 4140 MRB III, 465 21st Ave. South, Vanderbilt University, Nashville, TN 37232

Publication History

  1. Issue published online: 11 JAN 2005
  2. Article first published online: 11 JAN 2005
  3. Manuscript Accepted: 25 SEP 2004
  4. Manuscript Received: 12 JUL 2004

Funded by

  • Mouse Models of Human Cancers Consortium
  • GI Special Program of Research Excellence
  • VA Merit Review. Grant Numbers: CA46413, DK061218

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Keywords:

  • colon development;
  • colon cancer;
  • HES1;
  • Sox 4;
  • microarray

Abstract

During late embryogenesis, the mouse colon develops from a pseudostratified, undifferentiated endoderm to a single-layered columnar epithelium with accompanying mesenchymal maturation. To identify regulatory genetic programs underlying these morphological changes, we profiled gene expression of the developing mouse colon by microarray from embryonic day (E)13.5 to E18.5. Unbiased cluster analysis of 13,484 cDNA elements revealed two distinct groups of genes whose expression changes reflect the dynamic morphological events of the epithelium and mesenchyme during this period. Additional analyses revealed two subsets of genes whose expression is either upregulated or downregulated over the same developmental period. Of those genes whose expression increases from E13.5 to E18.5 (n = 158), known functions include acquisition and/or maintenance of colonic differentiation. Genes whose transcription is downregulated over this period (n = 49) have demonstrated roles in nuclear organization, transcriptional regulation, and cell proliferation. These results provide the basis for a molecular portrait of colonic development during late embryogenesis and should be a valuable resource for investigators interested in colonic development and neoplasia, as well as comparative organogenesis. genesis 41:1–12, 2005. © 2005 Wiley-Liss, Inc.

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