Y. Grinblat and M. Austen contributed equally to this work.
Combined haploid and insertional mutation screen in the zebrafish
Article first published online: 9 DEC 2004
Copyright © 2004 Wiley-Liss, Inc.
Volume 40, Issue 4, pages 231–240, December 2004
How to Cite
Wiellette, E., Grinblat, Y., Austen, M., Hirsinger, E., Amsterdam, A., Walker, C., Westerfield, M. and Sive, H. (2004), Combined haploid and insertional mutation screen in the zebrafish. Genesis, 40: 231–240. doi: 10.1002/gene.20090
- Issue published online: 9 DEC 2004
- Article first published online: 9 DEC 2004
- Manuscript Accepted: 2 OCT 2004
- Manuscript Received: 30 SEP 2004
- National Institutes of Health (NIH). Grant Numbers: AR45575, HD22486, NIH HD08704, MH059942-04
- Charles King Trust
- Deutsche Forschungsgemeinschaft
- insertional mutagenesis;
To identify genes required for development of the brain and somites, we performed a pilot screen of gynogenetic haploid zebrafish embryos produced from mothers mutagenized by viral insertion. We describe an efficient method to identify new mutations and the affected gene. In addition, we report the results of a small-scale screen that identified five genes required for brain development, including novel alleles of nagie oko, pou5f1, ribosomal protein L36, and n-cadherin, as well as a novel allele of the laminin g1 gene that is required for normal skeletal muscle fiber organization and somite patterning. genesis 40:231–240, 2004. © 2004 Wiley-Liss, Inc.