Germline transgenesis of the ascidian Ciona intestinalis by electroporation
Article first published online: 28 JAN 2005
Copyright © 2005 Wiley-Liss, Inc.
Volume 41, Issue 2, pages 67–72, February 2005
How to Cite
Matsuoka, T., Awazu, S., Shoguchi, E., Satoh, N. and Sasakura, Y. (2005), Germline transgenesis of the ascidian Ciona intestinalis by electroporation. Genesis, 41: 67–72. doi: 10.1002/gene.20096
- Issue published online: 14 FEB 2005
- Article first published online: 28 JAN 2005
- Manuscript Accepted: 30 OCT 2004
- Manuscript Received: 25 AUG 2004
- Ministry of Education, Culture, Sports, Science and Technology Japan. Grant Numbers: Grants-in-Aid 1600873, 0200967
- Japan Science and Technology Agency (Core Research for Evolutional Science and Technology program)
- transgenic technique;
- stable transgenic line;
- multiple copies of transgenes;
Microinjection of the Minos transposon is the only reported technique for generating stable transgenic lines in the cosmopolitan ascidian, Ciona intestinalis. To establish a more amenable method for generating stable transgenic Ciona, we examined the possibility of using electroporation of DNA into eggs. From 0–44.4% of electroporated individuals transmitted transgenes to the next generation. The transgene was integrated into one chromosome and multiple copies of the transgene were inserted into one site of the chromosome, indicating that electroporation is an easy and powerful technique for achieving stable transgenesis in C. intestinalis. Together with possible inland culture of this ascidian, this technique will be useful for generating stable lines which have reporter gene expression in a specific tissue or organ and the generation of transposase-expressing stable transgenic (jump-starter) lines and mutator lines which contain a lot of Minos transposons in an insertion position. genesis 41:67–72, 2005. © 2005 Wiley-Liss, Inc.