Synergistic activity of Sef and Sprouty proteins in regulating the expression of Gbx2 in the mid-hindbrain region

Authors

  • Wei Lin,

    1. Medical Research Council, National Institute for Medical Research, London, UK
    2. Shanghai Institutes for Biological Sciences, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China
    3. IGBMC, CNRS/INSERM/ULP, Collège de France, Strasbourg, France
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  • Naihe Jing,

    1. Shanghai Institutes for Biological Sciences, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China
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  • M. Albert Basson,

    1. Division of Hematology/Oncology, Department of Medicine, Mount Sinai School of Medicine, New York, New York
    Current affiliation:
    1. MRC Centre for Developmental Neurobiology, King's College London, London SE1 1UL, UK
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  • Andrée Dierich,

    1. IGBMC, CNRS/INSERM/ULP, Collège de France, Strasbourg, France
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  • Jonathan Licht,

    1. Division of Hematology/Oncology, Department of Medicine, Mount Sinai School of Medicine, New York, New York
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  • Siew-Lan Ang

    Corresponding author
    1. Medical Research Council, National Institute for Medical Research, London, UK
    2. IGBMC, CNRS/INSERM/ULP, Collège de France, Strasbourg, France
    • Division of Developmental Neurobiology, National Institute for Medical Research, The Ridgeway, Mill Hill, NW7 1AA London, UK
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Abstract

Sef and Sprouty proteins function as feedback antagonists of fibroblast growth factor (Fgf) signaling in zebrafish embryos. To study the role of Sef in mice, we generated Sef homozygous mutant animals. These animals are viable and show normal expression of mid-hindbrain genes at embryonic days 8.5 and 9.5. To investigate the possibility of functional synergism between Sef and Sprouty proteins, we electroporated Sprouty2Y55A, which functions in a dominant-negative manner in tissue culture cells into the mid-hindbrain region of wildtype and Sef mutant embryos. The expression pattern of Gbx2, a downstream target of Fgf signaling, was expanded or shifted in electroporated embryos, and this effect was significantly enhanced in the Sef mutant background. Altogether, our results demonstrate that Sef and Sproutys function synergistically to regulate Gbx2 expression in the anterior hindbrain. genesis 41:110–115, 2005. © 2005 Wiley-Liss, Inc.

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