Technology Report

Transgenic expression of Cre recombinase in mitral/tufted cells of the olfactory bulb

  1. Yumiko Nagai1,2,
  2. Hiromi Sano1,2,
  3. Mineto Yokoi1,2,*

Article first published online: 16 AUG 2005

DOI: 10.1002/gene.20146

Issue

genesis

genesis

Volume 43, Issue 1, pages 12–16, September 2005

Additional Information

How to Cite

Nagai, Y., Sano, H. and Yokoi, M. (2005), Transgenic expression of Cre recombinase in mitral/tufted cells of the olfactory bulb. Genesis, 43: 12–16. doi: 10.1002/gene.20146

Author Information

  1. 1

    Molecular Neurogenetics Unit, HMRO, Kyoto University Graduate School of Medicine, Kyoto, Japan

  2. 2

    CREST, Japan Science and Technology Agency, Kawaguchi, Japan

*Molecular Neurogenetics Unit, HMRO, Kyoto University Graduate School of Medicine, Yoshida-Konoe, Sakyo, Kyoto 606-8501, Japan

Publication History

  1. Issue published online: 16 AUG 2005
  2. Article first published online: 16 AUG 2005
  3. Manuscript Accepted: 15 MAY 2005
  4. Manuscript Received: 29 MAR 2005

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Keywords:

  • olfactory bulb;
  • mitral/tufted cell;
  • Cre recombinase;
  • transgenic mouse;
  • Pcdh21 promoter

Abstract

Olfactory information is conveyed from the periphery to the olfactory cortices through mitral and tufted (M/T) cells in the olfactory bulb. A mouse with a specific expression of Cre recombinase in M/T cells is essential for genetic marking of M/T cells and manipulating their properties. Protocadherin 21 (Pcdh21) expression is highly restricted to M/T cells. Here we report a transgenic mouse line, Pcdh21-Cre, in which ˜10-kb mouse Pcdh21 promoter drives the expression of Cre recombinase. In Pcdh21-Cre mice, Cre recombinase activity is predominantly detected in M/T cells, visualized with the anti-CFP immunostaining in offspring of a cross between Pcdh21-Cre and the reporter Rosa26-loxP-stop-loxP-CFP strain. These results demonstrate that the ˜10-kb Pcdh21 promoter can drive transcription in M/T cells and Pcdh21-Cre mice can be used to excise floxed DNA fragments in M/T cells, which provides a valuable tool to reveal the structure and function of the central olfactory circuits. genesis 43:12–16, 2005. © 2005 Wiley-Liss, Inc.

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