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Article
The Drosophila ACP65A cuticle gene: Deletion scanning analysis of cis-regulatory sequences and regulation by DHR38
Article first published online: 16 AUG 2005
DOI: 10.1002/gene.20150
Copyright © 2005 Wiley-Liss, Inc.
Additional Information
How to Cite
Bruey-Sedano, N., Alabouvette, J., Lestradet, M., Hong, L., Girard, A., Gervasio, E., Quennedey, B. and Charles, J.P. (2005), The Drosophila ACP65A cuticle gene: Deletion scanning analysis of cis-regulatory sequences and regulation by DHR38. Genesis, 43: 17–27. doi: 10.1002/gene.20150
Publication History
- Issue published online: 16 AUG 2005
- Article first published online: 16 AUG 2005
- Manuscript Accepted: 30 MAY 2005
- Manuscript Received: 23 SEP 2004
Funded by
- Burgundy region and the Centre National de la Recherche Scientifique
- French Ministère de l'Education Nationale, de l'Enseignement Supérieur et de la Recherche
- Association pour la Recherche contre le Cancer
- Abstract
- References
- Cited By
Keywords:
- Drosophila;
- cuticle;
- ecdysteroids;
- juvenile hormones;
- NGFI-B;
- DHR38;
- ACP65A;
- epidermis
Abstract
The regulatory sequences of the Drosophila ACP65A cuticle gene were analyzed in vivo in transgenic flies, using both fusion genes constructs and transposase-mediated deletions within a P element containing ACP65A regulatory sequences fused to the lacZ gene (deletion scanning). The sequences located between −594 and +161 are sufficient to confer both temporal and spatial expression specificities, indicating the presence of tissue-specific enhancers and response elements to hormone-induced factors. In addition, timing of expression and tissue-specificity appear to be controlled by distinct cis-regulatory elements, which suggests the existence of independent hormonal and tissue-specific signaling pathways. Gain and loss of function studies also implicate DHR38, the Drosophila homolog of the vertebrate NGFI-B-type nuclear receptors, as an important activator of the ACP65A gene. genesis 43:17–27, 2005. © 2005 Wiley-Liss, Inc.