Technology Report

Generation and characterization of the PKCγ-Cre mouse line

  1. Yu-Qiang Ding1,2,
  2. Chuan-Xi Xiang1,
  3. Zhou-Feng Chen1,*

Article first published online: 16 AUG 2005

DOI: 10.1002/gene.20151

Issue

genesis

genesis

Volume 43, Issue 1, pages 28–33, September 2005

Additional Information

How to Cite

Ding, Y.-Q., Xiang, C.-X. and Chen, Z.-F. (2005), Generation and characterization of the PKCγ-Cre mouse line. Genesis, 43: 28–33. doi: 10.1002/gene.20151

Author Information

  1. 1

    Departments of Anesthesiology, Psychiatry, Molecular Biology, and Pharmacology, School of Medicine,Washington University Pain Center, St. Louis, Missouri

  2. 2

    Laboratory of Neural Development, Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, P.R. China

*Departments of Anesthesiology, School of Medicine, Washington University Pain Center, St. Louis, MO 63110

Publication History

  1. Issue published online: 16 AUG 2005
  2. Article first published online: 16 AUG 2005
  3. Manuscript Accepted: 12 JUN 2005
  4. Manuscript Received: 20 APR 2005

Funded by

  • National Institutes of Health (NIH). Grant Number: R01 NS43968-01

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Keywords:

  • PKCγ;
  • gene targeting;
  • Cre;
  • brain;
  • spinal cord

Abstract

PKCgγ is a protein kinase C (PKC) isoform that is expressed in the central nervous system (CNS). We generated a PKCgγ-Cre mouse line and characterized the expression and activity of the Cre protein. Our studies show that Cre expression largely recapitulates the endogenous expression of PKCgγ. Several major sites of Cre expression are the cerebral cortex, hippocampus, thalamus, amygdala, and spinal cord. Examination of PKCgγ-Cre/ROSA26 mice reveals a similar X-gal staining pattern in the CNS, indicating that Cre recombinase is capable of removing LoxP sites in vivo. These data indicate that the PKCgγ-Cre mouse line could be a useful reagent for generating Cre-mediated tissue-specific knockouts in the CNS. genesis 43:28–33, 2005. © 2005 Wiley-Liss, Inc.

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