Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells
Article first published online: 6 JAN 2006
Copyright © 2006 Wiley-Liss, Inc.
Volume 44, Issue 1, pages 23–28, January 2006
How to Cite
Beard, C., Hochedlinger, K., Plath, K., Wutz, A. and Jaenisch, R. (2006), Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Genesis, 44: 23–28. doi: 10.1002/gene.20180
- Issue published online: 6 JAN 2006
- Article first published online: 6 JAN 2006
- Manuscript Accepted: 5 NOV 2005
- Manuscript Received: 15 SEP 2005
- embryonic stem cells;
- inducible gene expression;
- site specific recombination;
- FLPe recombinase
Transgenic and gene-targeted mutant mice provide powerful tools for analysis of the cellular processes involved in early development and in the pathogenesis of many diseases. Here we describe a transgene integration strategy mediated by site-specific recombination that allows establishment of multiple embryonic stem (ES) cell lines carrying tetracycline-inducible genes targeted to a specific locus to assure predictable temporal and spatial expression in ES cells and mice. Using homologous recombination we inserted an frt homing site into which tetracycline-inducible transgenes can be integrated efficiently in the presence of FLPe recombinase. This strategy and the vectors described here are generally applicable to any locus in ES cells and should allow for the rapid production of mice with transgenes efficiently targeted to a defined site. genesis 44:23–28, 2006. © 2006 Wiley-Liss, Inc.