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Keywords:

  • restriction fragment length polymorphism (RFLP);
  • insulin gene 5′ flanking polymorphism (5′ FP);
  • insulin gene 6.0-kb probe;
  • Southern blotting;
  • HLA-DBβ band patterns;
  • HLA-DQβ band patterns;
  • HLA-DR subtypes

Abstract

The primary aim of the insulin-dependent diabetes mellitus (IDDM) component of Genetic Analysis Workshop 5 (GAW5) was to collect and analyze new data on DNA polymorphisms closely linked to the HLA-D region and the insulin gene. The probes and restriction enzymes described here were used by all ten participating labs, and the data from Southern blotting were interpreted and reported according to conventions developed for the Workshop. These DNA data on members of 94 families with two or more IDDM sibs constitute the largest such sample available. The data were used in most of the analyses presented at the Workshop meeting, and are available on request.