Neurons modulate oxytocin receptor expression in rat cultured astrocytes: Involvement of TGF-β and membrane components
Article first published online: 12 DEC 2001
Copyright © 2002 Wiley-Liss, Inc.
Volume 37, Issue 2, pages 169–177, February 2002
How to Cite
Mittaud, P., Labourdette, G., Zingg, H. and Guenot-Di Scala, D. (2002), Neurons modulate oxytocin receptor expression in rat cultured astrocytes: Involvement of TGF-β and membrane components. Glia, 37: 169–177. doi: 10.1002/glia.10029
- Issue published online: 12 DEC 2001
- Article first published online: 12 DEC 2001
- Manuscript Accepted: 24 OCT 2001
- Manuscript Received: 13 AUG 2001
- CNRS UMR 7519
- University of Louis Pasteur, Strasbourg France
- rat astrocytes;
- neuron–glia interactions;
- RT PCR;
We examined the effect of neurons on oxytocin (OT) receptors (OTR) and OTR gene expression in cultured astrocytes. The addition of neuron-conditioned medium induced an increase of both OTR binding and OTR mRNA level. This effect was enhanced after the medium was boiled or acidified. As it is known that transforming growth factor-β (TGF-β) can be released from carrier proteins by acid or heat, TGF-β1 and 2 were tested and found to induce an increase of OTR binding. Furthermore, TGF-β antibody abolished the stimulatory effect of normal or acidified neuron-conditioned medium. Neurons added to cultured astrocytes without contact mimicked the stimulatory effect of the conditioned medium. In contrast, neurons added with contact, induced a decrease in OTR binding and an increase of mRNA level, whereas neuronal membranes induced a decrease of both OTR binding and mRNA levels. In conclusion, the present data demonstrate that in vitro, neurons are able to modulate astrocytic OTR expression at the level of both protein and mRNA. They stimulate this expression through their release of TGF-β and inhibit it by the action of unknown membrane components. GLIA 37:169–177, 2002. © 2002 Wiley-Liss, Inc.