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Cannabinoids ablate release of TNFα in rat microglial cells stimulated with lypopolysaccharide

  1. Fabrizio Facchinetti*,
  2. Elda Del Giudice,
  3. Sara Furegato,
  4. Marzla Passarotto,
  5. Alberta Leon

Article first published online: 28 DEC 2002

DOI: 10.1002/glia.10177

Issue

Glia

Glia

Volume 41, Issue 2, pages 161–168, 15 January 2003

Additional Information

How to Cite

Facchinetti, F., Del Giudice, E., Furegato, S., Passarotto, M. and Leon, A. (2003), Cannabinoids ablate release of TNFα in rat microglial cells stimulated with lypopolysaccharide. Glia, 41: 161–168. doi: 10.1002/glia.10177

Author Information

  1. Research & Innovation (R&I) Company, Padova, Italy

*Research & Innovation (R&I) Company, Via Svizzera 16, 35127 Padova, Italy

Publication History

  1. Issue published online: 28 DEC 2002
  2. Article first published online: 28 DEC 2002
  3. Manuscript Accepted: 13 SEP 2002
  4. Manuscript Received: 7 MAR 2002

Funded by

  • Italian Ministry of Education, University and Research Ministry (MIUR). Grant Number: 3933

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REFERENCES

  • Achiron A, Miron S, Lavie V, Margalit R, Biegon A. 2000. Dexanabinol (HU-211) effect on experimental autoimmune encephalomyelitis: implications for the treatment of acute relapses of multiple sclerosis. J Neuroimmunol 102: 26–31.
  • Axelrod J, Felder CC. 1998. Cannabinoid receptors and their endogenous agonist, anandamide. Neurochem Res 23: 575–581.
  • Bayewitch M, Avidor-Reiss T, Levy R, Barg J, Mechoulam R, Vogel Z. 1995. The peripheral cannabinoid receptor: adenylate cyclase inhibition and G protein coupling. FEBS Lett 375: 143–147.
  • Breivogel CS, Griffin G, Di Marzo V, Martin BR. 2001. Evidence for a new G protein-coupled cannabinoid receptor in mouse brain. Mol Pharmacol 60: 155–163.
  • Calandra B, Portier M, Kerneis A, Delpech M, Carillon C, Le Fur G, Ferrara P, Shire D. 1999. Dual intracellular signaling pathways mediated by the human cannabinoid CB1 receptor. Eur J Pharmacol 374: 445–455.
  • De Petrocellis L, Melck D, Bisogno T, Di Marzo V. 2000. Endocannabinoids and fatty acid amides in cancer, inflammation and related disorders. Chem Phys Lipids 108: 191–209.
  • Di Marzo V, Melck D, Bisogno T, De Petrocellis L. 1998. Endocannabinoids: endogenous cannabinoid receptor ligands with neuromodulatory action. Trends Neurosci 21: 521–528.
  • Felder CC, Veluz JS, Williams HL, Briley EM, Matsuda LA. 1992. Cannabinoid agonists stimulate both receptor- and non-receptor-mediated signal transduction pathways in cells transfected with and expressing cannabinoid receptor clones. Mol Pharmacol 42: 838–845.
  • Galiegue S, Mary S, Marchand J, Dussossoy D, Carriere D, Carayon P, Bouaboula M, Shire D, Le Fur G, Casellas P. 1995. Expression of central and peripheral cannabinoid receptors in human immune tissues and leukocyte subpopulations. Eur J Biochem 232: 54–61.
    Direct Link:
  • Gallily R, Breuer A, Mechoulam R. 2000. 2-arachidonylglycerol, an endogenous cannabinoid, inhibits tumor necrosis factor-alpha production in murine macrophages, and in mice. Eur J Pharmacol 406: R5–R7.
  • Gatley SJ, Gifford AN, Volkow ND, Lan R, Makriyannis A. 1996. 123I-labeled AM251: a radioiodinated ligand which binds in vivo to mouse brain cannabinoid CB1 receptors. Eur J Pharmacol 307: 331–338.
  • Gehrmann J, Matsumoto Y, Kreutzberg GW. 1995. Microglia: intrinsic immuneffector cell of the brain. Brain Res Brain Res Rev 20: 269–287.
  • Glass M, Felder CC. 1997. Concurrent stimulation of cannabinoid CB1 and dopamine D2 receptors augments cAMP accumulation in striatal neurons: evidence for a Gs linkage to the CB1 receptor. J Neurosci 17: 5327–5333.
  • Haslam RJ, Davidson MM, Desjardins JV. 1978. Inhibition of adenylate cyclase by adenosine analogues in preparations of broken and intact human platelets: evidence for the unidirectional control of platelet function by cyclic AMP. Biochem J 176: 83–95.
  • Heneka MT, Loschmann PA, Gleichmann M, Weller M, Schulz JB, Wullner U, Klockgether T. 1998. Induction of nitric oxide synthase and nitric oxide-mediated apoptosis in neuronal PC12 cells after stimulation with tumor necrosis factor-alpha/lipopolysaccharide. J Neurochem 71: 88–94.
    Direct Link:
  • Kaminski NE. 1998. Inhibition of the cAMP signaling cascade via cannabinoid receptors: a putative mechanism of immune modulation by cannabinoid compounds. Toxicol Lett 102/103: 59–63.
  • Kassiotis G, Bauer J, Akassoglou K, Lassmann H, Kollias G, Probert L. 1999. A tumor necrosis factor-induced model of human primary demyelinating diseases develops in immunodeficient mice. Eur J Immunol 29: 912–917.
    Direct Link:
  • Leker RR, Shohami E, Abramsky O, Ovadia H. 1999. Dexanabinol: a novel neuroprotective drug in experimental focal cerebral ischemia. J Neurol Sci 162: 114–119.
  • Levi G, Patrizio M, Bernardo A, Petrucci TC, Agresti C. 1993. Human immunodeficiency virus coat protein gp120 inhibits the beta-adrenergic regulation of astroglial and microglial functions. Proc Natl Acad Sci USA 90: 1541–1545.
  • Matsuda LA, Lolait SJ, Brownstein MJ, Young AC, Bonner TI. 1990. Structure of a cannabinoid receptor and functional expression of the cloned cDNA. Nature 346: 561–564.
  • Merrill JE, Benveniste EN. 1996. Cytokines in inflammatory brain lesions: helpful and harmful. Trends Neurosci 19: 331–338.
  • Molina-Holgado F, Lledo A, Guaza C. 1997. Anandamide suppresses nitric oxide and TNF-alpha responses to Theiler's virus or endotoxin in astrocytes. Neuroreport 8: 1929–1933.
  • Munro S, Thomas KL, Abu-Shaar M. 1993. Molecular characterization of a peripheral receptor for cannabinoids. Nature 365: 61–65.
  • Puffenbarger RA, Boothe AC, Cabral GA. 2000. Cannabinoids inhibit LPS-inducible cytokine mRNA expression in rat microglial cells. Glia 29: 58–69.
    Direct Link:
  • Rinaldi-Carmona M, Le Duigou A, Oustric D, Barth F, Bouaboula M, Carayon P, Casellas P, Le Fur G. 1998. Modulation of CB1 cannabinoid receptor functions after a long-term exposure to agonist or inverse agonist in the Chinese hamster ovary cell expression system. J Pharmacol Exp Ther 287: 1038–1047.
  • Sagan S, Venance L, Torrens Y, Cordier J, Glowiski J Giaume C. 1999. Anandamide and Win 555121-2 inhibit cyclic AMP formation through G-protein–coupled receptors distinct from CB1 cannabinoid receptors in cultured astrocytes. Eur J Neurosci 11: 691–699.
    Direct Link:
  • Sanchez C, Rueda D, Segui B, Galve-Roperh I, Levade T, Guzman M. 2001. The CB(1) cannabinoid receptor of astrocytes is coupled to sphingomyelin hydrolysis through the adaptor protein fan. Mol Pharmacol 59: 955–959.
  • Shohami E, Gallily R, Mechoulam R, Bass R, Ben-Hur T. 1997. Cytokine production in the brain following closed head injury: dexanabinol (HU-211) is a novel TNF-alpha inhibitor and an effective neuroprotectant. J Neuroimmunol 72: 169–177.
  • Sinha D, Bonner TI, Bhat NR, Matsuda LA. 1998. Expression of the CB1 cannabinoid receptor in macrophage-like cells from brain tissue: immunochemical characterization by fusion protein antibodies. J Neuroimmunol 82: 13–21.
  • Venters HD, Dantzer R, Kelley KW. 2000. Tumor necrosis factor-alpha induces neuronal death by silencing survival signals generated by the type I insulin-like growth factor receptor. Ann NY Acad Sci 917: 210–220.
    Direct Link:
  • Waksman Y, Olson JM, Carlisle SJ, Cabral GA. 1999. The central cannabinoid receptor (CB1) mediates inhibition of nitric oxide production by rat microglial cells. J Pharmacol Exp Ther 288: 1357–1366.
  • Zielasek J, Hartung HP. 1996. Molecular mechanisms of microglial activation. Adv Neuroimmunol 6: 191–122.
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