Slit and glypican-1 mRNAs are coexpressed in the reactive astrocytes of the injured adult brain
Article first published online: 6 MAR 2003
Copyright © 2003 Wiley-Liss, Inc.
Volume 42, Issue 2, pages 130–138, 15 April 2003
How to Cite
Hagino, S., Iseki, K., Mori, T., Zhang, Y., Hikake, T., Yokoya, S., Takeuchi, M., Hasimoto, H., Kikuchi, S. and Wanaka, A. (2003), Slit and glypican-1 mRNAs are coexpressed in the reactive astrocytes of the injured adult brain. Glia, 42: 130–138. doi: 10.1002/glia.10207
- Issue published online: 6 MAR 2003
- Article first published online: 6 MAR 2003
- Manuscript Accepted: 16 DEC 2002
- Manuscript Received: 19 AUG 2002
- Ministry of Education, Culture, Sports, Science, and Technology, Tokyo, Japan
- reactive astrocyte;
- brain injury;
The slit family serves as a repellent for growing axons toward correct targets during neural development. A recent report describes slit mRNAs expressed in various brain regions in adult rats. However, their functions in the adult nervous system remain unknown. In the present study, we investigated whether slit mRNAs were expressed in the cryo-injured brain, using in situ hybridization. All slit family members were expressed at the lesion. Slit2 mRNA was the most intensely expressed in the cells surrounding the necrotic tissue. A double-labeling study showed that slit2 mRNA was expressed in the glial fibrillary acidic protein (GFAP)-positive reactive astrocytes. In addition, glypican-1, a heparan sulfate proteoglycan that serves as a high-affinity receptor for Slit protein, was coexpressed with slit2 mRNA in the reactive astrocytes. These findings suggested that slit2 might prevent regenerating axons from entering into the lesion in concert with glypican-1. GLIA 42:130–138, 2003. © 2003 Wiley-Liss, Inc.