Inducible gene deletion in astroglia and radial glia—A valuable tool for functional and lineage analysis

Authors

  • Tetsuji Mori,

    1. Institute of Stem Cell Research, GSF-National Research Center for Environment and Health, Neuherberg/Munich, Germany
    Search for more papers by this author
  • Kohichi Tanaka,

    1. Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute,Tokyo Medical and Dental University, Tokyo, Japan
    Search for more papers by this author
  • Annalisa Buffo,

    1. Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute,Tokyo Medical and Dental University, Tokyo, Japan
    Search for more papers by this author
  • Wolfgang Wurst,

    1. Institute of Developmental Genetics, GSF-National Research Center for Environment and Health, Neuherberg/Munich, Germany
    Search for more papers by this author
  • Ralf Kühn,

    1. Institute of Developmental Genetics, GSF-National Research Center for Environment and Health, Neuherberg/Munich, Germany
    Search for more papers by this author
  • Magdalena Götz

    Corresponding author
    1. Institute of Stem Cell Research, GSF-National Research Center for Environment and Health, Neuherberg/Munich, Germany
    2. Department of Physiology, Ludwig-Maximilians University, Munich, Germany
    • Institute of Stem Cell Research, GSF-National Research Center for Environment and Health, Ingolstädter Landstr.1, D85764 Neuherberg/Munich, Germany
    Search for more papers by this author

Abstract

Astrocytes are thought to play a variety of key roles in the adult brain, such as their participation in synaptic transmission, in wound healing upon brain injury, and adult neurogenesis. However, to elucidate these functions in vivo has been difficult because of the lack of astrocyte-specific gene targeting. Here we show that the inducible form of Cre (CreERT2) expressed in the locus of the astrocyte-specific glutamate transporter (GLAST) allows precisely timed gene deletion in adult astrocytes as well as radial glial cells at earlier developmental stages. Moreover, postnatal and adult neurogenesis can be targeted at different stages with high efficiency as it originates from astroglial cells. Taken together, this mouse line will allow dissecting the molecular pathways regulating the diverse functions of astrocytes as precursors, support cells, repair cells, and cells involved in neuronal information processing. © 2006 Wiley-Liss, Inc.

Ancillary