These authors contributed equally to this work.
p38 mitogen-activated protein kinase is required for central nervous system myelination
Article first published online: 29 AUG 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 55, Issue 15, pages 1531–1541, 15 November 2007
How to Cite
Fragoso, G., Haines, J. D., Roberston, J., Pedraza, L., Mushynski, W. E. and Almazan, G. (2007), p38 mitogen-activated protein kinase is required for central nervous system myelination. Glia, 55: 1531–1541. doi: 10.1002/glia.20567
- Issue published online: 17 SEP 2007
- Article first published online: 29 AUG 2007
- Manuscript Accepted: 18 JUL 2007
- Manuscript Revised: 12 JUL 2007
- Manuscript Received: 6 APR 2007
- Multiple Sclerosis Society of Canada (MSSC)
- p38 MAPK;
- dorsal root ganglion neuron
The p38 MAPKs are a family of kinases that regulate a number of cellular functions including cell migration, proliferation, and differentiation. Here, we report that p38 regulates oligodendrocyte differentiation. Inhibition of p38 with PD169316 and SB203580 prevented accumulation of protein and mRNA of cell-stage specific markers characteristic of differentiated oligodendrocytes, including myelin basic protein, myelin-associated glycoprotein, and the glycosphingolipids, galactosylceramide and sulfatide. In addition, the cell cycle regulator p27kip1 and the transcription factor Sox10 were also significantly reduced. Most significantly, p38 inhibitors completely and irreversibly blocked myelination of dorsal root ganglion neurons by oligodendrocytes and prevented the axolemmal organization of the axo-glial adhesion molecule Caspr. Our results suggest a role(s) for this kinase in key regulatory steps in the maturation of OLGs and initiation of myelination. © 2007 Wiley-Liss, Inc.