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Expression of distinct splice variants of the stem cell marker prominin-1 (CD133) in glial cells

Authors

  • Denis Corbeil,

    Corresponding author
    1. Tissue Engineering Laboratories, BIOTEC and DFG Research Center and Cluster of Excellence for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Tatzberg 47-49, 01307 Dresden, Germany
    • Tissue Engineering Laboratories, BIOTEC, Technical University of Dresden, Tatzberg 47-49, D-01307 Dresden, Germany
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    • Denis Corbeil and Angret Joester contributed equally to this work.

  • Angret Joester,

    1. Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany
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    • Denis Corbeil and Angret Joester contributed equally to this work.

  • Christine A. Fargeas,

    1. Tissue Engineering Laboratories, BIOTEC and DFG Research Center and Cluster of Excellence for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Tatzberg 47-49, 01307 Dresden, Germany
    2. Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany
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  • József Jászai,

    1. Tissue Engineering Laboratories, BIOTEC and DFG Research Center and Cluster of Excellence for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Tatzberg 47-49, 01307 Dresden, Germany
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  • Jeremy Garwood,

    1. Department of Neurobiology, University of Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany
    Current affiliation:
    1. LNDR, CNRS, Centre de Neurochimie, 5 rue Blaise Pascal, 67084 Strasbourg Cedex, France
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  • Andrea Hellwig,

    1. Department of Neurobiology, University of Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany
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  • Hauke B. Werner,

    1. Zentrum für Molekulare Biologie, University of Heidelberg, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany
    Current affiliation:
    1. Max-Planck-Institute of Experimental Medicine, Hermann-Rein-Strasse 3, D-37075 Göttingen, Germany
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  • Wieland B. Huttner

    Corresponding author
    1. Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany
    • Max-Planck-Institute of Molecular, Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany
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Abstract

Prominin-1 (CD133) is a cholesterol-interacting pentaspan membrane glycoprotein specifically associated with plasma membrane protrusions. Prominin-1 is expressed by various stem and progenitor cells, notably neuroepithelial progenitors found in the developing embryonic brain. Here, we further investigated its expression in the murine brain. Biochemical analyses of brain membranes at early stages of development revealed the expression of two distinct splice variants of prominin-1, s1 and s3, which have different cytoplasmic C-terminal domains. The relative abundance of the s3 variant increased toward adulthood, whereas the opposite was observed for the s1 variant. Our combined in situ hybridization and immunohistochemistry revealed the expression of prominin-1 in a subpopulation of Olig-2-positive oligodendroglial cells present within white matter tracts of postnatal and adult brain. Furthermore, immunohistological and biochemical characterization suggested strongly that the s3 variant is a novel component of myelin. Consistent with this, the expression of prominin-1.s3 was significantly reduced in the brain of myelin-deficient mice. Finally, oligodendrocytes expressed selectively the s3 variant whereas GFAP-positive astrocytes expressed the s1 variant in primary glial cell cultures derived from embryonic brains. Collectively, our data demonstrate a complex expression pattern of prominin-1 molecules in developing adult brain. Given that prominin-1 is thought to act as an organizer of plasma membrane protrusions, they further suggest that a specific prominin-1 splice variant might play a role in morphogenesis and/or maintenance of the myelin sheath. © 2008 Wiley-Liss, Inc.

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