Original Article

Role of astroglial connexin30 in hippocampal gap junction coupling

  1. Dominic Gosejacob1,†,
  2. Pavel Dublin1,†,
  3. Peter Bedner1,
  4. Kerstin Hüttmann1,
  5. Jiong Zhang1,
  6. Oliver Tress2,
  7. Klaus Willecke2,
  8. Frank Pfrieger3,
  9. Christian Steinhäuser1,*,
  10. Martin Theis1,*

Article first published online: 29 DEC 2010

DOI: 10.1002/glia.21120

Issue

Glia

Glia

Volume 59, Issue 3, pages 511–519, March 2011

Additional Information

How to Cite

Gosejacob, D., Dublin, P., Bedner, P., Hüttmann, K., Zhang, J., Tress, O., Willecke, K., Pfrieger, F., Steinhäuser, C. and Theis, M. (2011), Role of astroglial connexin30 in hippocampal gap junction coupling. Glia, 59: 511–519. doi: 10.1002/glia.21120

Author Information

  1. 1

    Institute of Cellular Neurosciences, Medical Faculty, University of Bonn, 53105 Bonn, Germany

  2. 2

    Institute of Genetics, University of Bonn, 53117 Bonn, Germany

  3. 3

    CNRS UPR 3212, University of Strasbourg, Institute of Cellular and Integrative Neurosciences (INCI), 5, rue Louis Pasteur, 67084 Strasbourg Cedex, France

  1. †

    Dominic Gosejacob and Pavel Dublin contributed equally to this work.

*Institute of Cellular Neurosciences, Medical Faculty, University of Bonn, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany

Publication History

  1. Issue published online: 24 JAN 2011
  2. Article first published online: 29 DEC 2010
  3. Manuscript Accepted: 11 NOV 2010
  4. Manuscript Received: 7 SEP 2010

Funded by

  • DFG. Grant Numbers: SFB/TR3, C1, C9, N01, KE 329/23-1, SPP1172, SE 774/3, SPP1172 TH 1350/1-1
  • EC. Grant Number: FP7-202167 NeuroGLIA

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Keywords:

  • astrocytes;
  • Cx30;
  • intercellular communication;
  • transgenic;
  • reporter gene

Abstract

The impact of connexin30 (Cx30) on interastrocytic gap junction coupling in the normal hippocampus is matter of debate; reporter gene analyses indicated a weak expression of Cx30 in the mouse hippocampus. In contrast, mice lacking connexin43 (Cx43) in astrocytes exhibited only 50% reduction in coupling. Complete uncoupling of hippocampal astrocytes in mice lacking both Cx30 and Cx43 suggested that Cx30 participates in interastrocytic gap junction coupling in the hippocampus. With comparative reporter gene assays, immunodetection, and cre/loxP-based reporter approaches we demonstrate that Cx30 is more abundant than previously thought. The specific role of Cx30 in interastrocytic coupling has never been investigated. Employing tracer coupling analyses in acute slices of Cx30 deficient mice here we show that Cx30 makes a substantial contribution to interastrocytic gap junctional communication in the mouse hippocampus. © 2010 Wiley-Liss, Inc.

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