Dominic Gosejacob and Pavel Dublin contributed equally to this work.
Role of astroglial connexin30 in hippocampal gap junction coupling
Article first published online: 29 DEC 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 59, Issue 3, pages 511–519, March 2011
How to Cite
Gosejacob, D., Dublin, P., Bedner, P., Hüttmann, K., Zhang, J., Tress, O., Willecke, K., Pfrieger, F., Steinhäuser, C. and Theis, M. (2011), Role of astroglial connexin30 in hippocampal gap junction coupling. Glia, 59: 511–519. doi: 10.1002/glia.21120
- Issue published online: 24 JAN 2011
- Article first published online: 29 DEC 2010
- Manuscript Accepted: 11 NOV 2010
- Manuscript Received: 7 SEP 2010
- DFG. Grant Numbers: SFB/TR3, C1, C9, N01, KE 329/23-1, SPP1172, SE 774/3, SPP1172 TH 1350/1-1
- EC. Grant Number: FP7-202167 NeuroGLIA
- intercellular communication;
- reporter gene
The impact of connexin30 (Cx30) on interastrocytic gap junction coupling in the normal hippocampus is matter of debate; reporter gene analyses indicated a weak expression of Cx30 in the mouse hippocampus. In contrast, mice lacking connexin43 (Cx43) in astrocytes exhibited only 50% reduction in coupling. Complete uncoupling of hippocampal astrocytes in mice lacking both Cx30 and Cx43 suggested that Cx30 participates in interastrocytic gap junction coupling in the hippocampus. With comparative reporter gene assays, immunodetection, and cre/loxP-based reporter approaches we demonstrate that Cx30 is more abundant than previously thought. The specific role of Cx30 in interastrocytic coupling has never been investigated. Employing tracer coupling analyses in acute slices of Cx30 deficient mice here we show that Cx30 makes a substantial contribution to interastrocytic gap junctional communication in the mouse hippocampus. © 2010 Wiley-Liss, Inc.