Additional Supporting Information may be found in the online version of this article.

GLIA_21122_sm_SuppFig1.tif2802KSupporting Information Figure 1. Dilution of agents administered into the lateral ventricle and developmental changes in the periventricular white matter. (A) Mouse pups aged P8-P10 were injected with 86 μg/ml of LiCl into the lateral ventricle (V, inset) and lithium was measured in the periventricular white matter (PVWM, inset) using Atomic Absorption Spectroscopy at the time intervals indicated (arrows). Results are means ± SEM (n=3); inset illustrates a section immunolabelled for myelin basic protein (MBP) from the region of the posterior lateral ventricle analysed. (B) Confocal micrographs of the periventriclur corpus callosum from PLP/DsRed mice, at P8 and P11, and following treatment with saline/DMSO, and immunolabelled for MBP and PDGFαR, as indicated; scale bar represents 20 μm in top panels and 10 μm in lower panels.
GLIA_21122_sm_SuppFig2.tif6482KSupporting Information Figure 2. Lack of effect of ARA-014418 on axons, neurons and astrocytes. Mice aged P8 were injected twice daily for 3 days with saline/DMSO vehicle in controls or the GSK3β inhibitor ARA-014418, and brains were examined at P11 by coronal sections of periventricular forebrain. (A) Immunostaining for the mature axon marker NF200 illustrates no visible alterations in axon density after treatment with ARA-014418 in the corpus callosum. (B) Immunostaining for NeuN and GFAP for neuronal nuclei and astrocytes, respectively, in the periventricular cortex. Flattened confocal images are of 10 μm thickness. Scale bar represents 10 μm. (C) Quantification of GFAP+ astrocytes and NeuN+ neurons. Data are mean number of cells in a constant volume (FOV), as detailed in methods; error bars represent s.e.m.; n>4 animals (**P〈0.01 Dunnett's Multiple Comparisons test, followed by Bonferroni's posthoc test).
GLIA_21122_sm_SuppMaterials.doc209KSupporting Information

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