Astrocytic tissue remodeling by the meningitis neurotoxin pneumolysin facilitates pathogen tissue penetration and produces interstitial brain edema
Version of Record online: 11 OCT 2011
Copyright © 2011 Wiley Periodicals, Inc.
Volume 60, Issue 1, pages 137–146, January 2012
How to Cite
Hupp, S., Heimeroth, V., Wippel, C., Förtsch, C., Ma, J., Mitchell, T. J. and Iliev, A. I. (2012), Astrocytic tissue remodeling by the meningitis neurotoxin pneumolysin facilitates pathogen tissue penetration and produces interstitial brain edema. Glia, 60: 137–146. doi: 10.1002/glia.21256
- Issue online: 14 NOV 2011
- Version of Record online: 11 OCT 2011
- Manuscript Accepted: 21 SEP 2011
- Manuscript Received: 12 JUL 2011
- Emmy Noether Programme of the German Science Foundation (DFG), Würzburg. Grant Number: A.I. IL-151.1
- Rudolf Virchow Center for Experimental Medicine, Würzburg
- University of Würzburg, Würzburg
- Wellcome Trust, Glasgow
- Glasgow and European Science Foundation, Glasgow
Additional Supporting Information may be found in the online version of this article.
|GLIA_21256_sm_SuppFig1.tif||123K||Supporting Information Figure 1. Supernatant fluorescence intensity measurement after bacterial centrifugation and following a various number of washing of the bacterial suspension. At the given settings, the bacterial suspension demonstrated fluorescence of about 40, 000 A.U.|
|GLIA_21256_sm_SuppFig2.tif||2505K||Supporting Information Figure 2. Distribution of Evans blue dye (1%, 10 μL, i.c.v.) after 2 h in the brain dorsally (A), ventrally (B) and after a partial unfolding of the right hemisphere to visualize the ventricle (C). Abbreviations are as follows: Cx - cortex, (l) left and (r) right; S - brain stem; C - cerebellum, HTh - hypothalamus; OCh - optical chiasm; V - ventricle. D. Dilution of Evans blue dye in the CSF 2 h after injection and extrapolation to the equivalent PLY concentrations (pre-injection stock 25 μg/mL). E. Determination of the PLY concentration in the CSF of animals after Western blot analysis compared with serial dilutions 2 h after injection.|
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