Additional Supporting Information may be found in the online version of this article.

GLIA_22271_sm_SuppFig1.doc172KSupporting Information Figure 1. SDF-1-induced chemotaxis of astrocytes is mediated by CXCR7. (A, B) Cortical rat astrocytes were transfected overnight with either selective CXCR7 siRNA (A) or selective CXCR4 siRNA (B) and analyzed for CXCR7 and CXCR4 expression, respectively, 72 h post-transfection. GAPDH served as loading control. (C) Analysis of astrocytes with RNAi-mediated inhibition of CXCR7 or CXCR4 expression for SDF-1-induced chemotaxis using a modified Boyden chamber. The number of spontaneously migrating cells present in transfection reagent-pretreated (control) cultures was set to 1. Transfection with CXCR7 siRNA, but not with CXCR4 siRNA prevented SDF-1-induced chemotaxis. Data represent mean + SD from 3 independent experiments. *p<0.05, **p<0.001; ANOVA with Student-Newman-Keuls; absence vs. presence of SDF-1.
GLIA_22271_sm_SuppFig2.doc2473KSupporting Information Figure 2. SDF-1-dependent activation of Akt and Erk in human glioma cells is sensitive to CCX771. Confluent cultures of human A764 glioma cells were maintained for 6 h with serum-free N2-medium and subsequently stimulated for 15 min with the indicated concentrations of SDF-1 in absence or presence of the CXCR7 antagonist, CCX771. Activation (phosphorylation) of Akt and Erk was assessed by Western blotting using phospho-specific antibodies. To control for protein loading, blot were additionally stained with antibodies recognizing Akt and Erk independent of their phosphorylation status. CCX771 prevented SDF-1-dependent activation of Akt and Erk, hence, confirming that activation of both signalling molecules occurs through CXCR7.
GLIA_22271_sm_SuppTab1.doc255KSupporting Information Table 1. Quantification of Western blot analysis. Integrated optical densities of immunoreactive protein bands were measured using the Gel-Pro Analyzer software. Data were normalized to the respective loading control. One way analysis of variance (ANOVA) followed by pairwise multiple comparison procedures (Student-Newman-Keuls method) was used for statistical analysis. Differences with p<0.05 were considered significant.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.