Lin Xiao and Dazhi Guo contributed equally to this work.
Diosgenin promotes oligodendrocyte progenitor cell differentiation through estrogen receptor-mediated ERK1/2 activation to accelerate remyelination
Article first published online: 27 MAR 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 60, Issue 7, pages 1037–1052, July 2012
How to Cite
Xiao, L., Guo, D., Hu, C., Shen, W., Shan, L., Li, C., Liu, X., Yang, W., Zhang, W. and He, C. (2012), Diosgenin promotes oligodendrocyte progenitor cell differentiation through estrogen receptor-mediated ERK1/2 activation to accelerate remyelination. Glia, 60: 1037–1052. doi: 10.1002/glia.22333
- Issue published online: 8 MAY 2012
- Article first published online: 27 MAR 2012
- Manuscript Accepted: 1 MAR 2012
- Manuscript Received: 13 JAN 2012
- National Key Basic Research Program. Grant Number: 2011CB504401
- National Natural Science Foundation of China;. Grant Numbers: 30900431, 31130024
- Ministry of Science and Technology of China;. Grant Number: 2009ZX09311-001
Additional Supporting Information may be found in the online version of this article.
|GLIA_22333_sm_SuppFig1.tif||1218K||Supporting Information Figure 1. Purity of cultured OPCs identified by immunostaining. Isolated OPCs were cultured in B104 condition medium for 48hr, immunostained with A2B5 or NG2 and counterstained with Hoechst33342. More than 95% of the total cells were A2B5 or NG2 positive. Bar=20µm.|
|GLIA_22333_sm_SuppFig2.tif||1048K||Supporting Information Figure 2. Purity of cultured OPCs identified by immunostaining. OPCs cultures were maintained in B104 condition medium for 48hr, and then immunostained with Iba1(microglia cell maker) or GFAP (astrocyte cell maker). DIC (left) and fluorescence (middle and right) images showing the absence of Iba1 or GFAP positive cell in these cultures. Bar=20µm.|
|GLIA_22333_sm_SuppFig3.tif||1025K||Supporting Information Figure 3. OPCs are nestin positive. Isolated OPCs were cultured in B104 condition medium for 48hr and double immunostained with A25B and nestin. Corresponding DIC and fluorescence images were shown. Bar=20µm.|
|GLIA_22333_sm_SuppFig4.tif||1762K||Supporting Information Figure 4. Differentiation of OPCs in vitro. Up panel: phase images showing bipolar OPCs maintained in proliferation (prolif) medium; middle up panel: phase images showing differentiated OPCs after 3 days in differentiation (diff) medium; middle low: phase and fluorescence images showing two typical differentiated OPCs (mature oligodendroctye) which are MBP positive; low: representative immnuostaining of O4 and O1 of the OL cells in OPCs cultures after 3 days in differentiation medium. Bar=20µm.|
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