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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
GLIA_22333_sm_SuppFig1.tif1218KSupporting Information Figure 1. Purity of cultured OPCs identified by immunostaining. Isolated OPCs were cultured in B104 condition medium for 48hr, immunostained with A2B5 or NG2 and counterstained with Hoechst33342. More than 95% of the total cells were A2B5 or NG2 positive. Bar=20µm.
GLIA_22333_sm_SuppFig2.tif1048KSupporting Information Figure 2. Purity of cultured OPCs identified by immunostaining. OPCs cultures were maintained in B104 condition medium for 48hr, and then immunostained with Iba1(microglia cell maker) or GFAP (astrocyte cell maker). DIC (left) and fluorescence (middle and right) images showing the absence of Iba1 or GFAP positive cell in these cultures. Bar=20µm.
GLIA_22333_sm_SuppFig3.tif1025KSupporting Information Figure 3. OPCs are nestin positive. Isolated OPCs were cultured in B104 condition medium for 48hr and double immunostained with A25B and nestin. Corresponding DIC and fluorescence images were shown. Bar=20µm.
GLIA_22333_sm_SuppFig4.tif1762KSupporting Information Figure 4. Differentiation of OPCs in vitro. Up panel: phase images showing bipolar OPCs maintained in proliferation (prolif) medium; middle up panel: phase images showing differentiated OPCs after 3 days in differentiation (diff) medium; middle low: phase and fluorescence images showing two typical differentiated OPCs (mature oligodendroctye) which are MBP positive; low: representative immnuostaining of O4 and O1 of the OL cells in OPCs cultures after 3 days in differentiation medium. Bar=20µm.

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