Additional Supporting Information may be found in the online version of this article.

GLIA_22340_sm_SuppFig1.tif777KSupporting Information Figure 1. p38 and MK2 inhibitors reduce Sox10 and MBP accumulation. 10-d myelinating cultures treated with PD169316 (5 μM) or CMPD1 (2.5 μM) were harvested and analyzed by Western blotting. Membranes were incubated with antibodies to Sox10, MBP, and actin as a loading control. Representative blots (a) were quantified by densitometry (b) and corrected for equal loading. a, p<0.05 and b, p<0.001 from VC treated alone.
GLIA_22340_sm_SuppFig2.tif2777KSupporting Information Figure 2. p38 MAPK does not regulate Sox2 expression. Levels of Sox2, MBP and p27kip1 were assessed in myelinating co-cultures in the presence and absence of PD169316 (5 μM) using immunocytochemistry and immunoblotting. (a) Co-cultures were stimulated to myelinate with ECM for 3-d in the absence or presence of PD169316 (5 μM), and subsequently, immunolabeled for Sox2 (red) and p27kip1 (green). (d, e) VC-stimulated co-cultures treated with PD169316 (5 μM) were harvested after 10-d for immunoblotting, and quantified by densitometry. Results shown are from an experiment performed in duplicate.
GLIA_22340_sm_SuppFig3.tif3957KSupporting Information Figure 3. Inhibiting p38 MAPK in Schwann-cell DRGN co-cultures does not alter expression of Egr1 protein by p27kip1-expressing Schwann cells. 3-d VC-induced myelinating Schwann cell-DRGN co-cultures treated with PD169316 (5 μM) were immunostained for Egr1 (red) and p27kip1 (green). Schwann cell nuclei were visualized using DAPI (blue). Shown are representative confocal images: (a) control (no VC), (b) VC and (c) PD169316 in the presence of VC. Scale bar = 50 μm. Single p27kip1+ cells (white box) from VC- (b) and PD169316-treated cultures (c) are depicted at higher magnification in d-i (scale bar = 10 μm).

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