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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
GLIA_22370_sm_SuppFig1.tif54549KSupporting Information Figure 1. Gating strategy for ex vivo flow cytometric phenotyping of microglia from grey matter and white matter and macrophages from choroid plexus. Data were obtained from donor 10 (see table 1). FSC = forward scatter; SSC = side scatter
GLIA_22370_sm_SuppFig2.tif16238KSupporting Information Figure 2. M1 and M2 responses of human monocyte-derived macrophages. (A) Gene expression, as determined by qPCR analysis, after culture for 18 h with LPS alone, or in combination with IFN?. (B) Gene expression after culture for 72 h with IL-4. Note the marked upregulation of MR and CD200R. (C) Gene expression after culture for 72 h with dexamethasone. Note the limited upregulation of CCL18. Depicted are fold changes in mRNA levels after stimulation compared to those under basal culture conditions (n=2). Dotted lines indicate a two-fold change. Data shown represent median and interquartile range (donors not depicted in table 1). Dex = dexamethasone
GLIA_22370_sm_SuppFig3.tif29054KSupporting Information Figure 3. Strong enhancement of M1 responses in primary human microglia from a control donor after overnight culture. (A) Gene expression, as determined by qPCR analysis, in human WM microglia incubated for 4 h with LPS alone, or in combination with IFNγ, either immediately after isolation or after overnight culture in medium only. Note that after overnight, culture for 4 h with only LPS is enough to induce a marked upregulation of TNFα and CCR7, which is slightly augmented by the addition of IFN?. (B) Gene expression in human WM microglia incubated for 18 h with LPS alone, or in combination with IFNγ, either immediately after isolation or after overnight culture in medium only. Depicted are fold changes in mRNA levels after stimulation compared to those under basal culture conditions. Dotted lines indicate a two-fold change. Data shown represent median and interquartile range and were obtained from donor 24 (see table 1). Dex = dexamethasone
GLIA_22370_sm_SuppTab1.doc37KSupporting Information Table 1. Sequences of primers used for qPCR experiments

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