Oligodendroglia are limited in type I interferon induction and responsiveness in vivo
Article first published online: 26 JUN 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 60, Issue 10, pages 1555–1566, October 2012
How to Cite
Kapil, P., Butchi, N. B., Stohlman, S. A. and Bergmann, C. C. (2012), Oligodendroglia are limited in type I interferon induction and responsiveness in vivo. Glia, 60: 1555–1566. doi: 10.1002/glia.22375
- Issue published online: 16 AUG 2012
- Article first published online: 26 JUN 2012
- Manuscript Accepted: 31 MAY 2012
- Manuscript Received: 11 JAN 2012
- National Institute of Health. Grant Number: P01 NS064932
Additional Supporting Information may be found in the online version of this article.
|GLIA_22375_sm_SuppFig1.pdf||64K||Supporting Information Figure 1. Validation of minimum cell numbers required for reliable PCR products. Microglia from spinal cords of mice injected intracerebrally with poly I:C for 4 h were FACS purified. RNA was separately isolated from decreasing cell numbers as indicated and expression of Gapdh, Ifna4, Ifnb, Ifit1 and Ifit2 transcripts were analyzed. Data are representative of 10 pooled mice from a single experiment.|
|GLIA_22375_sm_SuppFig2.pdf||93K||Supporting Information Figure 2. Ex vivo stimulation of oligodendroglia and microglia with poly I:C or poly I:C supplemented with FuGENE. Purified microglia and CD45- O4+ oligodendroglia from spinal cords of naïve mice were stimulated with poly I:C only or poly I:C and FuGENE for 8 h and expression of Ifna4, Ifnβ, Ifit1, and Ifit2 transcripts were analyzed. Data are representative of two independent experiments each with 12 pooled mice per experiment.|
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