• Open Access

Expression profiling of Aldh1l1-precursors in the developing spinal cord reveals glial lineage-specific genes and direct Sox9-Nfe2l1 interactions

Authors

  • Anna V. Molofsky,

    1. Department of Pediatrics, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, California
    2. Department of Psychiatry, Langley Porter Psychiatric Institute, University of California San Francisco, San Francisco, California
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  • Stacey M. Glasgow,

    1. Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, Texas
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  • Lesley S. Chaboub,

    1. Program in Developmental Biology, Baylor College of Medicine, Houston, Texas
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  • Hui-Hsin Tsai,

    1. Department of Pediatrics, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, California
    2. Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California
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  • Alice T. Murnen,

    1. Department of Pediatrics, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, California
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  • Kevin W. Kelley,

    1. Department of Pediatrics, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, California
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  • Stephen P.J. Fancy,

    1. Department of Pediatrics, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, California
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  • Tracy J. Yuen,

    1. Department of Pediatrics, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, California
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  • Lohith Madireddy,

    1. Department of Neurology, University of California San Francisco, San Francisco, California
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  • Sergio Baranzini,

    1. Department of Neurology, University of California San Francisco, San Francisco, California
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  • Benjamin Deneen,

    1. Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, Texas
    2. Program in Developmental Biology, Baylor College of Medicine, Houston, Texas
    3. Department of Neuroscience, Baylor College of Medicine, Houston, Texas
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  • David H. Rowitch,

    Corresponding author
    1. Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California
    • Department of Pediatrics, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, California
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  • Michael C. Oldham

    1. Department of Neurology, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, California
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  • Anna V. Molofsky and Stacey M. Glasgow contributed equally.

Address correspondence to David H. Rowitch, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143. E-mail: RowitchD@peds.ucsf.edu and Michael C. Oldham, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143. E-mail: OldhamM@stemcell.ucsf.edu

Abstract

Developmental regulation of gliogenesis in the mammalian CNS is incompletely understood, in part due to a limited repertoire of lineage-specific genes. We used Aldh1l1-GFP as a marker for gliogenic radial glia and later-stage precursors of developing astrocytes and performed gene expression profiling of these cells. We then used this dataset to identify candidate transcription factors that may serve as glial markers or regulators of glial fate. Our analysis generated a database of developmental stage-related markers of Aldh1l1+ cells between murine embryonic day 13.5–18.5. Using these data we identify the bZIP transcription factor Nfe2l1 and demonstrate that it promotes glial fate under direct Sox9 regulatory control. Thus, this dataset represents a resource for identifying novel regulators of glial development.

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