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Blockade of microglial KATP-channel abrogates suppression of inflammatory-mediated inhibition of neural precursor cells

Authors

  • Francisco J. Ortega,

    1. Biochemistry and Molecular Biology Unit, School of Medicine, University of Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Barcelona, Spain
    2. Queensland Brain Institute, The University of Queensland, Brisbane, Queensland, Australia
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  • Jana Vukovic,

    1. Queensland Brain Institute, The University of Queensland, Brisbane, Queensland, Australia
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  • Manuel J. Rodríguez,

    1. Biochemistry and Molecular Biology Unit, School of Medicine, University of Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Barcelona, Spain
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  • Perry F. Bartlett

    Corresponding author
    1. Queensland Brain Institute, The University of Queensland, Brisbane, Queensland, Australia
    • Address correspondence to Perry F. Bartlett, Queensland Brain Institute, The University of Queensland, Brisbane, Queensland 4072, Australia. E-mail: p.bartlett@uq.edu.au

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Abstract

Microglia positively affect neural progenitor cell physiology through the release of inflammatory mediators or trophic factors. We demonstrated previously that reactive microglia foster KATP-channel expression and that blocking this channel using glibenclamide administration enhances striatal neurogenesis after stroke. In this study, we investigated whether the microglial KATP-channel directly influences the activation of neural precursor cells (NPCs) from the subventricular zone using transgenic Csf1r-GFP mice. In vitro exposure of NPCs to lipopolysaccharide and interferon-gamma resulted in a significant decrease in precursor cell number. The complete removal of microglia from the culture or exposure to enriched microglia culture also decreased the precursor cell number. The addition of glibenclamide rescued the negative effects of enriched microglia on neurosphere formation and promoted a ∼20% improvement in precursor cell number. Similar results were found using microglial-conditioned media from isolated microglia. Using primary mixed glial and pure microglial cultures, glibenclamide specifically targeted reactive microglia to restore neurogenesis and increased the microglial production of the chemokine monocyte chemoattractant protein-1 (MCP-1). These findings provide the first direct evidence that the microglial KATP-channel is a regulator of the proliferation of NPCs under inflammatory conditions. GLIA 2014;62:247–258

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