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glia22688-sup-0001-suppfig.tif3223KSupplementary Figure 1. Cells were selected with PDGFRα antibody from 16-19 gw human fetal brain tissues, initially cultured in DFM+PDGF-AA/bFGF for 4 days, and then changed to DFM +BDNF/IGF1 media. Cells were then treated with 50 nM FTY720-p. Media was changed and treatment repeated every 2 days for 6 days. Cultures were fixed at either 2 or 6 days and immunostained for O4 and either Ki67 (Panel A) or TUNEL (Panel B). Percentage of O4+Ki67+ cells (panel A) and percentage of O4+TUNEL+ cells (panel B) in each condition were quantified. Data represent 3 independent experiments performed in duplicate for each condition. Statistical significances were determined by two-way ANOVA, followed by Bonferroni post-tests. Comparison with control: *, p<0.05; **, p< 0.01; ***, p < 0.001.

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