Role of p38MAPK in S1P receptor-mediated differentiation of human oligodendrocyte progenitors
Article first published online: 9 MAY 2014
© 2014 Wiley Periodicals, Inc.
Volume 62, Issue 8, pages 1361–1375, August 2014
How to Cite
Cui, Q. L., Fang, J., Kennedy, T. E., Almazan, G. and Antel, J. P. (2014), Role of p38MAPK in S1P receptor-mediated differentiation of human oligodendrocyte progenitors. Glia, 62: 1361–1375. doi: 10.1002/glia.22688
- Issue published online: 14 JUN 2014
- Article first published online: 9 MAY 2014
- Manuscript Revised: 22 APR 2014
- Manuscript Accepted: 22 APR 2014
- Manuscript Received: 15 NOV 2013
- CIHR/Industry (Novartis) Program and the Multiple Sclerosis Society of Canada
Additional Supporting Information may be found in the online version of this article.
|glia22688-sup-0001-suppfig.tif||3223K||Supplementary Figure 1. Cells were selected with PDGFRα antibody from 16-19 gw human fetal brain tissues, initially cultured in DFM+PDGF-AA/bFGF for 4 days, and then changed to DFM +BDNF/IGF1 media. Cells were then treated with 50 nM FTY720-p. Media was changed and treatment repeated every 2 days for 6 days. Cultures were fixed at either 2 or 6 days and immunostained for O4 and either Ki67 (Panel A) or TUNEL (Panel B). Percentage of O4+Ki67+ cells (panel A) and percentage of O4+TUNEL+ cells (panel B) in each condition were quantified. Data represent 3 independent experiments performed in duplicate for each condition. Statistical significances were determined by two-way ANOVA, followed by Bonferroni post-tests. Comparison with control: *, p<0.05; **, p< 0.01; ***, p < 0.001.|
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