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Activation of sodium-dependent glutamate transporters regulates the morphological aspects of oligodendrocyte maturation via signaling through calcium/calmodulin-dependent kinase IIβ's actin-binding/-stabilizing domain

Authors

  • Zila Martinez-Lozada,

    1. Department of Anatomy and Neurobiology, Virginia Commonwealth University Medical Center, Richmond, Virginia
    2. Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México D.F., México
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    • Zila Martinez-Lozada and Christopher T. Waggener contributed equally to this work.

  • Christopher T. Waggener,

    1. Department of Anatomy and Neurobiology, Virginia Commonwealth University Medical Center, Richmond, Virginia
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    • Zila Martinez-Lozada and Christopher T. Waggener contributed equally to this work.

  • Karam Kim,

    1. Brain Science Institute, RIKEN, Wako, Saitama, Japan
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  • Shiping Zou,

    1. Department of Anatomy and Neurobiology, Virginia Commonwealth University Medical Center, Richmond, Virginia
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  • Pamela E. Knapp,

    1. Department of Anatomy and Neurobiology, Virginia Commonwealth University Medical Center, Richmond, Virginia
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  • Yasunori Hayashi,

    1. Brain Science Institute, RIKEN, Wako, Saitama, Japan
    2. Saitama University Brain Science Institute, Saitama University, Saitama, Japan
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  • Arturo Ortega,

    1. Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México D.F., México
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  • Babette Fuss

    Corresponding author
    1. Department of Anatomy and Neurobiology, Virginia Commonwealth University Medical Center, Richmond, Virginia
    • Address correspondence to Babette Fuss, Department of Anatomy and Neurobiology, Virginia Commonwealth University, PO Box 980709, Richmond, VA. E-mail: bfuss@vcu.edu

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Abstract

Signaling via the major excitatory amino acid glutamate has been implicated in the regulation of various aspects of the biology of oligodendrocytes, the myelinating cells of the central nervous system (CNS). In this respect, cells of the oligodendrocyte lineage have been described to express a variety of glutamate-responsive transmembrane proteins including sodium-dependent glutamate transporters. The latter have been well characterized to mediate glutamate clearance from the extracellular space. However, there is increasing evidence that they also mediate glutamate-induced intracellular signaling events. Our data presented here show that the activation of oligodendrocyte expressed sodium-dependent glutamate transporters, in particular GLT-1 and GLAST, promotes the morphological aspects of oligodendrocyte maturation. This effect was found to be associated with a transient increase in intracellular calcium levels and a transient phosphorylation event at the serine (S)371 site of the calcium sensor calcium/calmodulin-dependent kinase type IIβ (CaMKIIβ). The potential regulatory S371 site is located within CaMKIIβ's previously defined actin-binding/-stabilizing domain, and phosphorylation events within this domain were identified in our studies as a requirement for sodium-dependent glutamate transporter-mediated promotion of oligodendrocyte maturation. Furthermore, our data provide good evidence for a role of these phosphorylation events in mediating detachment of CaMKIIβ from filamentous (F)-actin, and hence allowing a remodeling of the oligodendrocyte's actin cytoskeleton. Taken together with our recent findings, which demonstrated a crucial role of CaMKIIβ in regulating CNS myelination in vivo, our data strongly suggest that a sodium-dependent glutamate transporter–CaMKIIβ–actin cytoskeleton axis plays an important role in the regulation of oligodendrocyte maturation and CNS myelination. GLIA 2014;62:1543–1558

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