SEARCH

SEARCH BY CITATION

Keywords:

  • Quisqualate;
  • Kainate;
  • Fura-2;
  • DiBa-C4-(3)

Abstract

Kainate-, quisqualate- and glutamate-induced depolarization and mobilization of intracellular Ca2+ was determined in primary cultured astrocytes using the fluorescent probes DiBa-C4-(3) and fura-2, respectively. All three receptor agonists depolarized the cells in a Na+-dependent manner and increased the intracellular Ca2+ concentration. The glutamate- and quisqualate-induced increase in cytosolic Ca2+ was only partially inhibited by removal of extracellular Ca2+, whereas the response to kainate was totally dependent on extracellular Ca2+. The mechanisms for depolarization and increases in cytosolic Ca2+ appeared to be independent of each other, as extracellular Ca2+ removal or intracellular Ca2+ buffering with entrapped BAPTA did not affect the depolarization. Removal of extracellular Na+ did not affect the agonist-induced increase in Ca2+. If quisqualate was added after kainate, the cells were hyperpolarized in a Ca2+- and K+-dependent manner. This could be due to effects on a Ca2+-dependent K+ channel, the effects of which are normally hidden by the greater effect on Na+ channels as a response to quisqualate.