Characterization of C6-10A glioma cells highly responsive to β-adrenergic receptor agonist-induced NGF synthesis/secretion
Version of Record online: 12 OCT 2004
Copyright © 1994 Wiley-Liss, Inc.
Volume 12, Issue 2, pages 151–160, September 1994
How to Cite
Fukumoto, H., Kakihana, M. and Suno, M. (1994), Characterization of C6-10A glioma cells highly responsive to β-adrenergic receptor agonist-induced NGF synthesis/secretion. Glia, 12: 151–160. doi: 10.1002/glia.440120209
- Issue online: 12 OCT 2004
- Version of Record online: 12 OCT 2004
- Manuscript Accepted: 9 JUN 1994
- Manuscript Received: 29 NOV 1993
- Neurotrophic factor;
A subline of rat C6 glioma cells, C6-10A cells, in which epinephrine can induce nerve growth factor (NGF) synthesis/secretion, was isolated. C6-10A cells have retained their sensitivity to epinephrine for more than 2 years in a medium containing 0.5% fetal calf serum (FCS) but easily lose it in 10% FCS. C6-10A cells are S-100- and glial fibrillary acidic protein-positive, and the doubling time is about 60 h in the medium containing 0.5% FCS and about 20 h in 10% FCS. Epinephrine induced NGF synthesis/secretion prominently in serum-free cultures of C6-10A cells and in cultures with a high cell density, but not in serum-containing cultures. The induction did not occur with parent C6 cells under the appropriate conditions in C6-10A cells. NGF secretion was induced by catecholaminergic compounds in the following order isoproterenol > epinephrine = norepinephrine » dopamine. The induction caused by epinephrine was blocked by propranolol (α-blocker) but not by phentolamine (β-blocker). Various compounds that activate the adenylate cyclase system also induced NGF synthesis/secretion. These results indicate that C6-10A cells are astrocytes that are highly responsive to β-adrenergic receptor agonists, which stimulate NGF synthesis/secretion via receptors coupled with adenylate cyclase machinery. These cells may be a useful aid in studying the mechanism of NGF synthesis/secretion.