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Keywords:

  • Acute phase response;
  • Acute phase protein;
  • Alzheimer's disease;
  • Multiple sclerosis;
  • Microglia

Abstract

Neuropathological studies of the amyloid depositions and senile plaques in the brains of elderly patients or patients or patients diagnosed with Alzheimer's disease reveal the conspicuous presence of numerous proteins which are usually expressed during reactions of the immune system. This has led to speculations that the pathomechanism of neurodegenerative diseases might involve inflammatory processes. These considerations constitute the theoretical basis for therapeutic intervention with antiinflammatory drugs in neurodegenerative diseases.

Here, we show that the βA4-amyloid precursor (APP) is rapidly induced in microglia in a model of experimental antoimmune encephalomyelitis (EAE). Using specific monoclonal antibodies against APP, the first glial cells newly expressing APP immunoreactivity were found at an early preclinical stage, i.e., 24 h after T-cell transfer. At the peak of clinical disease (6 days after T-cell transfer), numerous characteristically ramified cells were strongly positive for APP. Based on morphology and double-labeling, most of the de novo APP-expressing cells were identified as microglia. Additionally, APP-immunoreactive round cells were detected in and around perivascular infiltrates. Reflecting the course of the clinical disease, the induction of APP-immunoreactivity terminated in the postclinical stage, i.e., 14 days after T-cell transfer.

These results support earlier work demonstration that microglia can rapidly de novo synthesise APP not in response to direct nerve injury (Banati et al: Glia 9:199, 1993a) but also in immune-mediated disease. Apart from ist possible therapeutic relevance, such a production of APP-reminiscent of an acute phase protein—suggests a role of APP in immune and repair mechanisms of the central nervous system. © 1995 Wiley-Liss, Inc.