Resting-state networks show dynamic functional connectivity in awake humans and anesthetized macaques

Authors

  • R. Matthew Hutchison,

    1. Graduate Program in Neuroscience, University of Western Ontario, London, Ontario, Canada
    2. Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada
    3. Robarts Research Institute, London, Ontario, Canada
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  • Thilo Womelsdorf,

    1. Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada
    2. Department of Biology, Centre for Vision Research, York University, Toronto, Ontario, Canada
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  • Joseph S. Gati,

    1. Robarts Research Institute, London, Ontario, Canada
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  • Stefan Everling,

    1. Graduate Program in Neuroscience, University of Western Ontario, London, Ontario, Canada
    2. Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada
    3. Robarts Research Institute, London, Ontario, Canada
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  • Ravi S. Menon

    Corresponding author
    1. Robarts Research Institute, London, Ontario, Canada
    • Graduate Program in Neuroscience, University of Western Ontario, London, Ontario, Canada
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Correspondence to: Ravi S. Menon, Robarts Research Institute, 100 Perth Drive, London, Ontario N6A 5K8, Canada. E-mail: rmenon@imaging.robarts.ca

Abstract

Characterization of large-scale brain networks using blood-oxygenation-level-dependent functional magnetic resonance imaging is typically based on the assumption of network stationarity across the duration of scan. Recent studies in humans have questioned this assumption by showing that within-network functional connectivity fluctuates on the order of seconds to minutes. Time-varying profiles of resting-state networks (RSNs) may relate to spontaneously shifting, electrophysiological network states and are thus mechanistically of particular importance. However, because these studies acquired data from awake subjects, the fluctuating connectivity could reflect various forms of conscious brain processing such as passive mind wandering, active monitoring, memory formation, or changes in attention and arousal during image acquisition. Here, we characterize RSN dynamics of anesthetized macaques that control for these accounts, and compare them to awake human subjects. We find that functional connectivity among nodes comprising the “oculomotor (OCM) network” strongly fluctuated over time during awake as well as anaesthetized states. For time dependent analysis with short windows (<60 s), periods of positive functional correlations alternated with prominent anticorrelations that were missed when assessed with longer time windows. Similarly, the analysis identified network nodes that transiently link to the OCM network and did not emerge in average RSN analysis. Furthermore, time-dependent analysis reliably revealed transient states of large-scale synchronization that spanned all seeds. The results illustrate that resting-state functional connectivity is not static and that RSNs can exhibit nonstationary, spontaneous relationships irrespective of conscious, cognitive processing. The findings imply that mechanistically important network information can be missed when using average functional connectivity as the single network measure. Hum Brain Mapp 34:2154–2177, 2013. © 2011 Wiley Periodicals, Inc.

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