Vision enhancement system for detection of oral cavity neoplasia based on autofluorescence
Article first published online: 27 FEB 2004
Copyright © 2004 Wiley Periodicals, Inc.
Head & Neck
Volume 26, Issue 3, pages 205–215, March 2004
How to Cite
Svistun, E., Alizadeh-Naderi, R., El-Naggar, A., Jacob, R., Gillenwater, A. and Richards-Kortum, R. (2004), Vision enhancement system for detection of oral cavity neoplasia based on autofluorescence. Head Neck, 26: 205–215. doi: 10.1002/hed.10381
- Issue published online: 27 FEB 2004
- Article first published online: 27 FEB 2004
- Manuscript Accepted: 25 AUG 2003
- Texas Higher Education Coordinating Board
- oral cancer;
- squamous cell carcinoma;
- fluorescence imaging;
Early detection of squamous cell carcinoma (SCC) in the oral cavity can improve survival. It is often difficult to distinguish neoplastic and benign lesions with standard white light illumination. We evaluated whether a technique that capitalizes on an alternative source of contrast, tissue autofluorescence, improves visual examination.
Autofluorescence of freshly resected oral tissue was observed visually and photographed at specific excitation/emission wavelength combinations optimized for response of the human visual system and tissue fluorescence properties. Perceived tumor margins were indicated for each wavelength combination. Punch biopsies were obtained from several sites from each specimen. Sensitivity and specificity were evaluated by correlating histopathologic diagnosis with visual impression.
Best results were achieved with illumination at 400 nm and observation at 530 nm. Here, sensitivity and specificity were 91% and 86% in discrimination of normal tissue from neoplasia. This compares favorably with white light examination, in which sensitivity and specificity were 75% and 43%.
Oral cavity autofluorescence can be easily viewed by the human eye in real time. Visual examination of autofluorescence enhances perceived contrast between normal and neoplastic oral mucosa in fresh tissue resections. © 2004 Wiley Periodicals, Inc. Head Neck26: 205–215, 2004