Upregulation of discoidin domain receptor 2 in nasopharyngeal carcinoma

Authors

  • Huey-Huey Chua PhD,

    1. Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Number 1, Jen-Ai Road, Section 1, Taipei 10051, Taiwan, Republic of China
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    • Huey-Huey Chua and Te-Huei Yeh contributed equally to this work.

  • Te-Huei Yeh MD,

    1. Department of Otolaryngology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Number 1, Jen-Ai Road, Section 1, Taipei 10051, Taiwan, Republic of China
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    • Huey-Huey Chua and Te-Huei Yeh contributed equally to this work.

  • Ying-Piao Wang MD,

    1. Department of Otolaryngology, Mackay Memorial Hospital, Number 92, Chung-Shan North Road, Section 2, Taipei 10449, Taiwan, Republic of China
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  • Yu-Tzu Huang PhD,

    1. Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Number 1, Jen-Ai Road, Section 1, Taipei 10051, Taiwan, Republic of China
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  • Tzung-Shiahn Sheen MD,

    1. Department of Otolaryngology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Number 1, Jen-Ai Road, Section 1, Taipei 10051, Taiwan, Republic of China
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  • You-Chang Lo BS,

    1. Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Number 1, Jen-Ai Road, Section 1, Taipei 10051, Taiwan, Republic of China
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  • Ya-Ching Chou MS,

    1. Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Number 1, Jen-Ai Road, Section 1, Taipei 10051, Taiwan, Republic of China
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  • Ching-Hwa Tsai PhD

    Corresponding author
    1. Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Number 1, Jen-Ai Road, Section 1, Taipei 10051, Taiwan, Republic of China
    • Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Number 1, Jen-Ai Road, Section 1, Taipei 10051, Taiwan, Republic of China
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  • Presented at the 12th Biennial Conference of the International Association for Research on Epstein-Barr Virus and Associated Diseases, Boston, USA, July 8–12, 2006.

Abstract

Background.

Nasopharyngeal carcinoma (NPC) is associated with Epstein-Barr virus (EBV) and has high metastatic potential. Discoidin domain receptors (DDR1, DDR2) are receptor-type tyrosine kinases activated by collagen. Their ability to induce expression of matrix metalloproteinase is related with tumor invasion. Therefore, we aim to investigate DDRs gene expression and its regulation in NPC.

Methods and Results.

By use of real-time quantitative polymerase chain reaction (Q-PCR), DDR2 gene expression but not DDR1 was significantly higher in primary and metastatic NPC. DDR2 was predominantly distributed in NPC tumor cells rather than in infiltrating lymphocytes. EBV Z-transactivator (Zta) transfection may distinctly elevate DDR2 level. Furthermore, data from reporter assay indicate that Zta could transactivate DDR2 promoter activity, suggesting the possible upregulation mechanism.

Conclusion.

DDR2 was differentially upregulated in NPC and modulated by EBV Zta protein. DDR2 may play a role in NPC invasion and serve as a diagnostic and therapeutic target. © 2007 Wiley Periodicals, Inc. Head Neck, 2008

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