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Expression of matrix metalloproteinase-1, -7, -9, -13, Ki-67, and HER-2 in epithelial-myoepithelial salivary gland cancer

Authors

  • Heikki Luukkaa MD,

    1. Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, University of Turku, Turku, Finland
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  • Pekka Klemi MD, PhD,

    1. Department of Pathology, University of Turku, Turku, Finland
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  • Ilmo Leivo MD, PhD,

    1. Haartman Institute, Department of Pathology, University of Helsinki, Helsinki University Central Hospital, Helsinki, Finland
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  • Antti A. Mäkitie MD, PhD,

    1. Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, University of Turku, Turku, Finland
    2. Department of Otorhinolaryngology - Head and Neck Surgery, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland
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  • Jonathan Irish MD, PhD,

    1. Departments of Otolaryngology - Head and Neck Surgery and Surgical Oncology, Princess Margaret Hospital, University Health Network, University of Toronto, Toronto, Ontario, Canada
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  • Ralph Gilbert MD, PhD,

    1. Departments of Otolaryngology - Head and Neck Surgery and Surgical Oncology, Princess Margaret Hospital, University Health Network, University of Toronto, Toronto, Ontario, Canada
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  • Bayardo Perez-Ordonez MD, PhD,

    1. Department of Pathology, University Health Network, University of Toronto, Toronto, Ontario, Canada
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  • Pirkko Hirsimäki MD, PhD,

    1. Department of Pathology, University of Turku, Turku, Finland
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  • Tero Vahlberg MSc,

    1. Department of Biostatistics, University of Turku, Turku, Finland
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  • Atte Kivisaari MD,

    1. Department of Dermatology, University of Turku, Turku, Finland
    2. Medicity Research Laboratory, University of Turku, Turku, Finland
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  • Veli-Matti Kähäri MD, PhD,

    1. Department of Dermatology, University of Turku, Turku, Finland
    2. Medicity Research Laboratory, University of Turku, Turku, Finland
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  • Reidar Grénman MD, PhD

    Corresponding author
    1. Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, University of Turku, Turku, Finland
    2. Department of Medical Biochemistry and Molecular Biology, University of Turku, Turku, Finland
    • Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital, University of Turku, Turku, Finland
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Abstract

Background

The expression of matrix metalloproteinases (MMPs) in epithelial-myoepithelial salivary gland carcinoma has not been studied previously.

Methods

Immunohistochemistry for MMP-1, -7, -9, -13, Ki-67, and HER-2, as well as HER-2 gene amplification by silver enhanced in situ hybridization was performed in a series of 12 paraffin-embedded histopathologic samples of patients from Canada and Finland.

Results

A positive MMP-13 (p = .0022), higher MMP-13 (p = .0274), and higher MMP-9 (p = .0274) index (multiplication of staining intensity by percentage of the positive cells) predicted better overall survival. In disease-specific analysis, higher MMP-9 index (p = .0327) predicted better survival. A higher volume corrected index (VCI) of Ki-67 (p = .0339) predicted worse disease-specific survival. In 1 patient, HER-2 oncogene amplification was observed.

Conclusion

MMPs and Ki-67 may have prognostic impact in epithelial-myoepithelial carcinoma. © 2009 Wiley Periodicals, Inc. Head Neck, 2010

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