Selective culture of different types of human parotid gland cells
Article first published online: 19 JUL 2010
Copyright © 2010 Wiley Periodicals, Inc.
Head & Neck
Volume 33, Issue 3, pages 407–414, March 2011
How to Cite
Chan, Y.-H., Huang, T.-W., Young, T.-H. and Lou, P.-J. (2011), Selective culture of different types of human parotid gland cells. Head Neck, 33: 407–414. doi: 10.1002/hed.21465
- Issue published online: 11 FEB 2011
- Article first published online: 19 JUL 2010
- Manuscript Accepted: 30 MAR 2010
- human parotid gland;
- primary culture;
- acinar cells;
- myoepithelial cells;
Advances in salivary gland tissue engineering can benefit patients diagnosed with xerostomia. Complexity of the gland explains the urgent demand for a reliable protocol to isolate and expand various gland cells that can be used for further study.
Three cells with different morphologies were isolated from the same human parotid glands using different culture medium systems and then were identified by the expressions from mRNA to the protein level.
Among the 34 specimens, parotid gland acinar cells, myoepithelial cells, and fibroblasts expressing specific markers that belonged to individual cell types, were successfully isolated and expanded from 30 specimens without a complex mechanical process and expensive flow technique.
The proposed protocol is simple with a high success rate to culture various gland cells, making it highly promising for use in future tissue engineering studies. © 2010 Wiley Periodicals, Inc. Head Neck, 2010