Resistance to apoptosis-inducing stimuli in CD44+ head and neck squamous cell carcinoma cells
Article first published online: 5 APR 2011
Copyright © 2011 Wiley Periodicals, Inc.
Head & Neck
Volume 34, Issue 3, pages 336–343, March 2012
How to Cite
Chikamatsu, K., Ishii, H., Takahashi, G., Okamoto, A., Moriyama, M., Sakakura, K. and Masuyama, K. (2012), Resistance to apoptosis-inducing stimuli in CD44+ head and neck squamous cell carcinoma cells. Head Neck, 34: 336–343. doi: 10.1002/hed.21732
- Issue published online: 7 FEB 2012
- Article first published online: 5 APR 2011
- Manuscript Accepted: 22 DEC 2010
- Manuscript Revised: 25 NOV 2010
- Manuscript Received: 10 AUG 2010
- Ministry of Education, Cultures, Sports, Science, and Technology of Japan. Grant Numbers: 20592013, 20592014
- cancer stem cells;
- head and neck squamous cell carcinoma;
- treatment resistance
CD44 was identified previously as a surface marker in cancer stem cells (CSCs) of head and neck squamous cell carcinoma (HNSCC). Most cancer treatments have been linked to the activation of the apoptosis-signaling pathway; however, the resistance mechanisms to apoptosis in CSCs have not yet been fully elucidated.
The sensitivity of CD44+ cells to diverse apoptosis-inducing stimuli was compared with that of CD44- cells. Furthermore, cell cycle changes and the expression of anti-apoptosis-related genes were examined using flow cytometry and real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR).
CD44+ cells were resistant to various apoptosis-inducing stimuli. Moreover, CD44+ cells showed a higher proportion of cells in G2/M phase of the cell cycle and upregulation of Bcl-2 and inhibitor of apoptosis (IAP) family genes compared with CD44- cells.
Treatment resistance in CSCs seems to be regulated by various mechanisms, and, therefore, additional treatment strategies to target CSCs are required in patients with HNSCC. © 2011 Wiley Periodicals, Inc. Head Neck, 2012