Effective treatment of 7,12-dimethylbenz(a)anthracene–induced hamster buccal pouch precancerous lesions by topical photosan-mediated photodynamic therapy

Authors

  • Chun-Pin Chiang DDS, DMSc,

    1. Graduate Institute of Oral Biology and Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan
    2. Department of Dentistry, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan
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  • Wan-Ting Huang MS,

    1. Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan, Taiwan
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  • Jeng-Woei Lee PhD,

    1. Department of Life Science, Tzu Chi University, Hualien, Taiwan
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  • Yih-Chih Hsu PhD

    Corresponding author
    1. Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan, Taiwan
    2. Center for Nanotechnology, Chung Yuan Christian University, Taoyuan, Taiwan
    • Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan, Taiwan
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Abstract

Background

Previous studies found that topical photodynamic therapy (PDT) is very effective for human oral precancerous lesions.

Methods

This study evaluated whether topical photosan-mediated PDT (topical photosan–PDT), using a 640-nm light-emitting diode (LED) light, is an effective treatment modality for hamster buccal pouch precancerous lesions. Fourteen 7,12-dimethylbenz(a)anthracene (DMBA)–induced hamster buccal pouch precancerous lesions were treated with topical photosan–PDT using the 640-nm LED light twice a week.

Results

All 14 of the precancerous lesions showed a complete histologically confirmed response to the lesions after an average of 3.79 (range, 3–5) PDT treatments. Normal and precancerous pouch mucosae in the other 4 hamsters received 17 or 19 treatments of topical photosan–PDT showed no cumulative side effects. No recurrence of the lesions was found in these 18 PDT-treated hamsters after a follow-up period of 50 weeks.

Conclusion

Our findings indicate that topical photosan–PDT is a very effective treatment modality for DMBA-induced hamster buccal pouch precancerous lesions. © 2011 Wiley Periodicals, Inc. Head Neck, 2012.

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